沙门氏菌、志贺氏菌和金黄色葡萄球菌共增菌培养基的研制  

An Enrichment Broth for Simultaneous Growth of Salmonella, Shigellosis and Staphylococcus aureus

作　　者：王永志[1] 余以刚[1] 黄秀丽[2] 肖性龙[1] 吴晖[1] 

机构地区：[1]华南理工大学食品安全与检测中心,广东广州510640 [2]广东省惠州市质量计量监督检测所,广东惠州516003

出　　处：《食品科学》2013年第17期171-175,共5页Food Science

基　　金：惠州市科技计划项目(0031939140712048);广东省科技计划项目(2011B020314004)

摘　　要：为研制出一种能够同时选择性富集沙门氏菌、志贺氏菌和金黄色葡萄球菌的培养基,对多种抑制剂和促进剂进行筛选。实验优化出的最佳培养基配方为:胰蛋白胨15g、大豆蛋白胨5g、磷酸二氢钾2.5g、葡萄糖2.5g、氯化钠35g、氯化锂0.5g、牛胆盐0.1g、甘露醇2g、亚碲酸钾0.3mg,蒸馏水1000mL。沙门氏菌、志贺氏菌和金黄色葡萄球菌等目标细菌在此共增菌培养基中均能快速生长,以103CFU/mL为初始菌浓,18h后菌浓能达到107CFU/mL,而非目标菌在此肉汤中的生长受到抑制。结果表明,此培养基可以用于沙门氏菌、志贺氏菌和金黄色葡萄球菌的选择性共增菌。In order to develop an enrichment broth for the simultaneous growth of Salmonella, Shigellosis and Staphylococcus, several candidate agents were added to pre-formulated broth to observe their growth speeds. The optimal performance was obtained with the enrichment broth composed of 15 g of tryptone, 5 g of soya peptone, 2.5 g of monopotassium phosphate, 2.5 g of glucose, 35 g of sodium chloride, 0.5 g of lithium chloride, 0.1 g of bile salt, 2 g of mannitol and 0.3 g of potassium tellurite in 1000 mL of distilled water. Results showed that Salmonella, Shigellosis and Staphylococcus aureus could concurrently enrich in this broth and the concentration of bacteria could increased from 103 CFU/mL to 107 CFU/mL during 18 h. The growth of non-target bacteria was suppressed in this broth. Therefore, it may be a promising broth for simultaneous detection of the three pathogens in a single platform.

关 键 词：选择性共增菌 沙门氏菌 志贺氏菌 金黄色葡萄球菌 

分 类 号：Q933[生物学—微生物学]