纤维素酶和碱性蛋白酶联合提取茶渣中蛋白质的工艺参数研究  

Optimizing process parameters for the extract of protein from tea residue by cellulose enzyme and alkaline protease

作　　者：于鹏亮[1] 李立祥[1] 卢昭[1] 张雁飞[1] 薛晨[1] 华再欣[2] 梅玉[3] 

机构地区：[1]安徽农业大学茶叶生物化学与生物技术教育部,农业部重点实验室,合肥230036 [2]安徽农业大学茶与食品科技学院,合肥230036 [3]安徽农业大学理学院,合肥230036

出　　处：《安徽农业大学学报》2013年第5期771-777,共7页Journal of Anhui Agricultural University

基　　金：安徽省高等学校"十五"优秀人才计划项目资助

摘　　要：研究纤维素酶和碱性蛋白酶联合作用提取绿茶茶渣中蛋白质的工艺。以提取温度、加酶量、pH值、提取时间为参考因素设计实验,响应面设计确定最佳提取条件。结果表明,纤维素酶酶解最佳工艺:温度41℃、加酶量2%、pH值6.4、时间64 min,蛋白质提取率6.58%。碱性蛋白酶酶解工艺:温度60℃、加酶量2%、pH值8.7、时间90 min,蛋白质提取率20.93%。最终双酶联合提取茶叶蛋白质的提取率为32.18%。The extraction process of protein from green tea residue by cellulose enzyme and alkaline protease was investigated. Reference factors including the extraction temperature, enzyme dosage, pH value and extraction time were considered to determine the best extraction condition by response surface design. The results showed that the optimum condition for protein extract with cellulose enzyme was 41℃, 2% cellulose enzyme, pH 6.4, and 64 min for enzymolysis. The protein extraction rate was 6.58% under the condition. The optimum condition for protein extract with alkaline protease was 60℃, 2% alkaline protease, pH 8.7, and 90 min for enzymolysis. The protein extraction rate was 20.93% under this condition, and the rate with combination of these two enzymes was 32.18%.

关 键 词：纤维素酶 碱性蛋白酶 茶渣 蛋白质 响应面设计 

分 类 号：TS272.2[农业科学—茶叶生产加工]