响应面法优化植物乳杆菌和凝结芽孢杆菌冻干保护剂的研究  

Optimization of Cryoprotectants for Bacillus Coagulans and Lactobacillus Plantarum by Response Surface Methods

作　　者：王世宽[1] 洪玉程[1] 袁先铃[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《四川理工学院学报（自然科学版）》2013年第5期23-26,共4页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：四川省教育厅项目(cc12z07);四川理工学院研究生创新基金项目(y2011022)

摘　　要：采用单因素实验和响应面实验设计联用的方法,筛选优化植物乳杆菌和凝结芽孢杆菌真空冷冻干燥的保护剂配方。单因素实验结果表明:脱脂奶粉、麦芽糖和谷氨酸钠3种保护剂效果较好,能够显著提高菌种对冷冻干燥环境的耐受能力。以细胞存活率为响应值,混合保护剂的最佳配方为脱脂奶粉7.06%、麦芽糖6.46%和谷氨酸钠6.70%,植物乳杆菌冷冻干燥存活率达到92.95%,凝结芽孢杆菌的冷冻干燥存活率达到94.1%。The formula of the freeze-dried protectants for Bacillus coagulans and Lactobacillus plantarum is optimized and selected by the method that combines single factor experiment and response surface experiment design. The single factor experiment shows that~ the skim milk, maltose and sodium glutamate have outstanding protective effect, which contribute to improve the cell＇s endurance capacity in freeze-dried environment. By response surface analysis, the quadratic model for the three significant factors is established with cell survival rate as the target response. The optima formula is： the skim milk 7.06% , maltose 6.46% and sodium glutamate 6. 70% , with which the cell survival rate for Lactobacillus plantarum reaches 92. 95 % and that for Bacillus coagulans reaches 94. 1%.

关 键 词：植物乳杆菌 凝结芽孢杆菌 真空冷冻干燥 保护剂 响应面法 

分 类 号：TS201.3[轻工技术与工程—食品科学]