铁皮石斛多糖提取工艺优化及其成分分析  

Optimized Extraction and Compositional Analysis of Polysaccharides from Dried Stems of Dendrobium officinale

作　　者：黄晓君[1] 聂少平[1] 王玉婷[1] 王雅薇[1] 蔡海兰[1] 田芳 崔武卫[1,3] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]云南金九地生物科技有限公司,云南昆明650033 [3]加拿大农业与农业食品部圭尔夫食品研究中心

出　　处：《食品科学》2013年第22期21-26,共6页Food Science

基　　金：国家自然科学基金重点项目(31130041);"十二五"国家科技支撑计划项目(2012BAD33B06);国家国际科技合作专项(2013DFA32710);教育部新世纪优秀人才支持计划项目(NCET-12-0749);江西省教育厅资助科研项目(KJLD13004)

摘　　要：采用响应面法优化铁皮石斛多糖的提取工艺,并对经优化提取得到的多糖基本理化性质进行分析。结果表明:铁皮石斛多糖提取的最佳工艺条件为水料比30:1(mL/g)、提取温度90℃、提取时间2h,在此条件下多糖得率为30.56%。铁皮石斛多糖中糖含量为78.21%、淀粉含量为18.64%、蛋白含量为5.8%、灰分含量为1.28%,主要由甘露糖、葡萄糖组成,两者的物质的量比为5.2:1。铁皮石斛多糖富含Ca(1.07%)、Mg(0.11%)、Fe(0.0012%)、Zn(0.0039%)等元素。高效凝胶渗透色谱结果显示铁皮石斛多糖呈单一色谱峰,均一性较好。通过本方法制得的铁皮石斛多糖的糖含量较高,除淀粉和蛋白外无其他含量较高的杂质成分;该多糖均一性较好,并且富含Ca、Mg、Fe、Zn等矿物质,研究成果可为日后铁皮石斛多糖活性研究以及构效关系讨论提供基础。Response surface methodology was utilized to optimize the extraction process of polysaccharides from dried stems of Dendrobium officinale （DOP）. The physiochemical properties of DOP obtained by the optimized process were analyzed. The optimal extraction conditions were found to be an extraction duration of 2 h at 90 ℃ with a water/material ratio of 30：1 （mL/g）. The yield of polysaccharides under the optimized conditions was 30.56%. In the resulting extract, 78.21% of neutral polysaccharide, 18.64% of starch, 5.8% of protein and 1.28% of ash were contained, with mannose and glucose being the major monosaccharides at a molar ratio of 5.2：1. DOP was rich in Ca （1.07%）, Mg （0.11%）, Fe （0.0012%） and Zn （0.0039%）. HPGPC analysis demonstrated that the polysaccharide was homogeneous with a single peak. This polysaccharide-rich extract may be useful for future studies on its activity and structure-activity relationship.

关 键 词：铁皮石斛多糖 提取工艺优化 响应面 成分分析 

分 类 号：TS201.1[轻工技术与工程—食品科学]