副溶血性弧菌生物被膜的形成与优化  

Research on the Formation and Optimization of Vibrio Parahaemolyticus Biofilm

作　　者：渠宏雁[1] 孟良玉[1] 刘锦峰[1] 蔡文倩[1] 卢佳琨[1] 张霞[1] 兰桃芳[1] 励建荣[1] 

机构地区：[1]渤海大学化学化工与食品安全学院辽宁省食品安全重点实验室辽宁省高校重大科技平台“食品贮藏加工及质量安全控制工程技术研究中心”辽宁锦州121013

出　　处：《中国食品学报》2013年第9期56-61,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十二五”国家科技支撑计划(2012BAD29B06);辽宁省食品安全重点实验室暨辽宁省高校重大科技平台“食品贮藏加工及质量安全控制工程技术研究中心”开放课题(LNSAKF2011020)

摘　　要：本文主要探讨了各种环境因子对副溶血性弧菌生物被膜形成的影响,旨在为以后控制细菌形成生物被膜提供依据。选用副溶血性弧菌为研究对象,采用微孔板检测法,用酶标仪测得的OD630值确定副溶血性弧菌生成生物被膜的最佳条件。试验结果表明：起始细菌浓度7 logcfu/mL、培养温度28℃、培养基NaCl含量3%、培养基起始pH 8~9时,测得的OD630值最大;培养基中分别加入蔗糖、乳糖、葡萄糖,OD630值均增加,且均在含量2%时促进效果最明显;培养基未稀释时,测得的OD630值最大,且随着培养基稀释度的增加而减小;培养基中添加Ca2＋促进生物被膜的形成,而添加Mg2＋、Cu2＋、Fe3＋对生物被膜的形成有抑制作用。副溶血性弧菌能形成稳定且明显的生物被膜,其生物被膜的形成受温度、NaCl浓度、pH值等环境因子的影响。Influence of Environmental factors on Vibrio parahaemolyticus biofilm formation was studied in this paper. The microtitre plate assay was used to determine the OD630 value to evaluate the optimal conditions for Vibrio para- haemolyticus biofilm growth. The results indicated that the maximal OD630 value was showed under the conditions： bacte- rial concentration with a density of 107cfu/mL, incubation temperature at 28 ℃, consistence of NaC1 at 3%, pH 8-9. It is most remarkable to impact on Vibrio parahaemolyticus biofilm formation in culture medium, which contains 2% su- crose, lactose and glucose, respectively. The OD630 value of bacterial biofilm reduced with the decrease in the concentra- tion of cultural media. Addition of Ca2＋ obviously promoted the bacterial biofilm formation, while Mg2＋, Fe3＋ and Cu2＋ played as opposite roles. Above all, the bacterial biofihn formation of Vibrio parahaemolyticus was govemable, which will be useful for the epidemic control in fish culture.

关 键 词：副溶血性弧菌 生物被膜 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]