电子舌用于药物掩味效果评价的研究  

Study on Application of Electronic Tongue in the Evaluation of Taste-masking Effect of Pharmaceutical Formulation

作　　者：李学林[1] 李慧玲[2] 刘瑞新[1] 张杏芬[2] 仇继玺[2] 吴子丹[2] 

机构地区：[1]河南中医学院第一附属医院,郑州450000 [2]河南中医学院药学院,郑州450008

出　　处：《世界科学技术-中医药现代化》2013年第7期1532-1537,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委青年基金项目(81001646):基于味觉指纹技术的中药汤剂苦味抑制剂掩味规律研究;负责人:刘瑞新

摘　　要：药物掩味效果评价对药学工作者开展药物掩味研究具有重要的意义。为了研究电子舌用于药物掩味效果评价的可行性,本实验利用盐酸小檗碱作为苦味模型药物,选择醋酸钠、2,4-二羟基甲苯酸、甜蜜素等作为苦味掩味物质,通过电子舌进行测定,根据主成分分析结果及苦距、苦度降低距、抑制率等指标对比,判断各掩味剂对盐酸小檗碱的掩味效果是否和志愿者口尝药物评判方法所得结果一致;并将掩味效果最好的甜蜜素配制成不同的浓度,对其用量进行优化。数据处理结果显示,在0.005 mg·mL-1的盐酸小檗碱溶液中,各掩味剂的掩味效果好坏依次为甜蜜素>2,4-二羟基苯甲酸>醋酸钠;随着甜蜜素用量的增加,其掩味效果越来越好,但0.2%以后的浓度掩味效果变化相对较小,在实际工作中,可以选择0.2%的浓度来掩味;本实验结果与口尝实验结果一致。本研究表明,在一定程度上,电子舌可作为评价药物掩味效果的一种有效的工具。Taste-masking effect of pharmaceutical formulation is important for the pharmacy worker to carry out study on taste-masking of pharmaceutical formulation. This study discussed the feasibility of electronic tongue in the evaluation of taste-masking effect. The berberine hydrochloride was used as model drug of bitterness. Sodium cycla-mate, 2,4-Dihydroxybenzoic acid and sodium cyclamate were used as bitterness inhibitors. Through detection of elec-tronic tongue, results from principal component analysis （PCA）, and indexes such as distance of bitterness （D）, dis-tance of bitterness reduction, inhibition rate （Ir）, the determination was given on whether results from the bitterness inhibition of berberine hydrochloride and taste results from volunteers were the same. In addition, sodium cyclamate, which was the best bitterness inhibition, was made into different concentrations in order to optimize its using dosage. The data analysis results showed that in the berberine hydrochloride solution with the concentration of 0.005 mg/mL, the ranking order of the bitterness-masking efficiency was sodium cyclamate 〉 2,4-Dihydroxybenzoic acid 〉 sodium acetate anhydrous. Along with the increasing concentration of sodium cyclamate, the taste-masking effect is better. However, after the concentration level of 0.2%, the change on taste-masking effect was relatively small. In the prac-tical work, the concentration of sodium cyclamate can be selected at the level of 0.2%. This experiment effect was the same as the human taste results. This study showed that the electronic tongue may be a useful tool in the evalua-tion of taste-masking efficiency to some extent.

关 键 词：电子舌 苦味 盐酸小檗碱 掩味 甜蜜素 主成分分析 苦距 口尝法 

分 类 号：R318.6[医药卫生—生物医学工程]