柱前衍生-反相高效液相色谱法研究稻谷中有机硒形态  

Determination of organic selenium compounds in rice by pre-column derivative-RP-HPLC method

作　　者：唐莹莹[1] 袁建[1] 蒋旭玲[1] 李倩[1] 

机构地区：[1]南京财经大学食品科学与工程学院,江苏南京210046

出　　处：《粮食与油脂》2013年第12期26-28,共3页Cereals & Oils

基　　金：江苏省科技自主创新项目CX(12)3086;江苏省产学研项目(BY2012037)

摘　　要：建立了稻谷中有机硒化合物测定方法，采用温和水解富硒大米蛋白的提取条件，利用柱前衍生～反相高效液相色谱测定水解大米蛋白中有机硒含量。结果表明：采用6mol／LHCl溶液、50℃水解富硒大米蛋白48h的条件提取率高。以4-氯-3，5-二硝基三氟甲苯（CNBF）为衍生剂的柱前衍生一反相HPLC检测富硒大米中有机硒化舍物主要是硒代甲硫氨酸（seMet），最低检出限为0．14μmol／L，线性范围为0．40~480I．tmol／L，加标回收率为89．9％～90．8％，硒代甲硫氨酸含量为4．122gg／g，占有机硒含量65．29％。该方法简便、灵敏、重现性好、干扰少，可为稻谷中有机硒测定提供一种完善检测方法。Abstract： This paper for the determination of organic selenium in rice by a mid hydrolysis and extraction method to determined by pre-column derivative-RP-HPLC. The results showed that the extraction rate with 100ml of 6mol/L HC1 solution, a constant temperature of 50 ℃, extraction time of 48 h was high. Method of pre-column derivatization-RP-HPLC for the detection of organic selenium compounds which 4-chlorine-3,5-dinitrobenzotrifluoride （ CNBF ） organic selenium compounds in selenium-enriched rice was mainly selenomethionine （ SeMet ）, The detection limit for selenium was 0.14 μtmol/L. The linear range was within 0.404480 pmol/L. The recovery was 89.9%～90.8%. contented for 4.122 gg/g selenium in protein, accounting for 65.29% of the selenium content. This method is was the derivatizing agent was simple, rapid and accurate.

关 键 词：有机硒 硒代氨基酸 柱前衍生一反相高效液相色谱法 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]