Effect of flupirtine on the growth and viability of U373 malignant glioma cells  

作　　者：Elango Panchanathan Gnanasambandan Ramanathan Bhaskar Venkata Kameswara Subrahmanya Lakkakula 

机构地区：[1]Department of Pharmacology,Sri Ramachandra University [2]Department of Biomedical Sciences,Sri Ramachandra University

出　　处：《Cancer Biology & Medicine》2013年第3期142-147,共6页癌症生物学与医学（英文版）

基　　金：supported by an intramural grant from Sri Ramachandra University,Chennai

摘　　要：Objective： Flupirtine is a non-opioid analgesic without antipyretic or antiphlogistic properties but with favorable tolerability in humans. ＂Ibis analgesic also exhibits neuroprotective activities. Furthermore, flupirtine antagonizes glutamate- and . , 2＋ NMDA-mduced mtracellular levels of Ca and counteracts the effects of focal cerebral Lscherma. Although fluplrtme has been used to relieve pain caused by different diseases and clinical procedures, information on the safety and efficacy of flupirtine is limited. ＂fhe present study was conducted to investigate the neuroprotective effects of flupirtine on U373 malignant glioma （MG） cell lines. Methods： Cellviability and cell cycle analysis was performed by MTF assay and flow cytomet-,＇y, respectively. Results： Variations in the growth of U373 MG cells in $ mM N-methyl-D-aspartate （NMDA）, 1 mM flupirtine, and combined treatment indicated the antagonistic effects of NMDA and flupirtine on MG cell lines. The variation in the percentage of gated cellpopulation in different cell cycle phases showed significant variations after 48 h of treatment. Conclusion： Flupirtine has neuroprotective effect of on U373 MG cells, which limits its use in the pain management of brain tumors. This property warrants further studies using animal models and large-scale clinical trials.Objective:Flupirtine is a non-opioid analgesic without antipyretic or antiphlogistic properties but with favorable tolerability in humans.This analgesic also exhibits neuroprotective activities.Furthermore,flupirtine antagonizes glutamateand NMDA-induced intracellular levels of Ca2+ and counteracts the effects of focal cerebral ischemia.Although flupirtine has been used to relieve pain caused by different diseases and clinical procedures,information on the safety and efficacy of flupirtine is limited.The present study was conducted to investigate the neuroprotective effects of flupirtine on U373 malignant glioma(MG) cell lines.Methods:Cell viability and cell cycle analysis was performed by MTT assay and flow cytometry,respectively.Results:Variations in the growth of U373 MG cells in 5 mM N-methyl-D-aspartate(NMDA),1 mM flupirtine,and combined treatment indicated the antagonistic effects of NMDA and flupirtine on MG cell lines.The variation in the percentage of gated cell population in different cell cycle phases showed significant variations after 48 h of treatment.Conclusion:Flupirtine has neuroprotective effect of on U373 MG cells,which limits its use in the pain management of brain tumors.This property warrants further studies using animal models and large-scale clinical trials.

关 键 词：FLUPIRTINE N-METHYLASPARTATE ANALGESICS GLIOMA 

分 类 号：R739.4[医药卫生—肿瘤]