葡萄酒中赭曲霉毒素A检测方法优化  

An Optimized Procedure for Rapid Determination of Ochratoxin A in Wine

作　　者：庞世琦[1] 刘青[1] 李志勇[1] 潘丙珍[1] 罗敏 刘茜[1] 陈文锐[1] 

机构地区：[1]广东检验检疫技术中心,广东广州510623 [2]南海出入境检验检疫局,广东佛山528200

出　　处：《食品科学》2013年第24期193-196,共4页Food Science

基　　金：广东省科技计划项目(2011B050400025;2010B020316006);质检公益性行业科研专项(201210104-2);广东检验检疫局科技计划项目(2013GDK31)

摘　　要：对葡萄酒中赭曲霉毒素A(OTA)的前处理和检测方法进行优化,分别采用液液萃取和免疫亲合柱前处理,结合高效液相色谱-荧光检测(HPLC-FD)和酶联免疫法检测(ELISA)对OTA的回收率、检测成本、检测效率及环保性进行比较,选出适用于大批量样品筛查的首选方法。同时针对目前大量进口的法国、西班牙、意大利、澳大利亚等国家以及国产的葡萄酒进行检测。结果表明:大批量葡萄酒样品的筛查建议采用液液萃取前处理结合ELISA方法进行检测的组合作为首选,检测到阳性样品时再用免疫亲和柱净化后用高效液相色谱检测进行确证,方法具有快捷、灵敏、准确等特点,回收率、精密度都能达到检测要求。The purpose of this study was to establish the optimal sample pretreatment procedure and analytical method for determining ochratoxin A （OTA） in wine. Sample pretreatment was achieved by alternative methods, either by liquid-liquid extraction or by immunoaffinity column chromatography （IAC） and two different analytical methods, high-performance liquid chromatography （HPLC） and enzyme-linked immunosorbent assay （ELISA）, were compared for their suitability for large-scale determination of OTA in wine from the viewpoints of OTA recovery, cost, efficiency and environmental protection. The selected analytical procedure was validated by applying it for the determination of wines imported from France, Spain, Italy and Australia as well as domestic wines. Based on the results of this study, liquid-liquid extraction in combination with ELISA is a more suitable method for large-scale screening of wines for OTA and can be validated by comparative determination of the positive samples using HPLC analysis following IAC clean-up. The method proposed in this study is rapid, convenient, sensitive and accurate and meets the analytical requirements of recovery and precision.

关 键 词：赭曲霉毒素A 葡萄酒 高效液相色谱法 酶联免疫吸附测定法 方法优化 

分 类 号：TS261.7[轻工技术与工程—发酵工程]