单宁酶固定化技术的研究  

Study on Tannase Immobilization Technology

作　　者：曹建兰[1,2] 吴鑫颖[1,3] 王晓丹[1,3] 周鸿翔[1,3] 邱树毅[1,3] 王广莉[1,3] 

机构地区：[1]贵州大学化学与化工学院,贵阳550025 [2]贵州遵义高等医科专业学校,遵义563000 [3]贵州省发酵工程与生物制药重点试验室,贵阳550025

出　　处：《食品工业》2014年第2期25-28,共4页The Food Industry

基　　金：贵州省科技攻关项目(黔科合GY字(2009)3022号);贵州省自然科学基金项目(黔科合J字[2008]2091号);贵州省自然科学基金项目(黔科合J字[2010]2279号)

摘　　要：采用交联+包埋+交联法对单宁酶进行固定化,在单因素的基础进行了正交优化研究,并对固定化单宁酶的最适作用温度、最适作用pH等酶学性质进行了初步研究。前交联戊二醛体积分数为0.3%,后交联戊二醛体积分数为0.4%,前交联温度为30℃,后交联时间1.0 h为最优条件。单宁酶经固定化后,温度和pH稳定性显著提高,固定化酶的最适作用温度为50℃,最适作用pH 5.0。Tannase was immobilized by the method of the pre cross-linking, embedding and re cross-linking. The orthogonal design is carried out on the basis of single factor, by which the optimum conditions of the immobilized tannase was selected out, the optimum pre cross-linking and re cross-linking volume fraction of glutaraldehyde is 0.3% and 0.4%, respectively. The pie cross-linking temperature is 30 °C and the re cross-linking time is 1.0 h. In addition, the optimum catalytic temperature is 50 °C and the optimum catalytic pH is 5.0, and compered with the free tannase, the temperature and the pH stability of immobilized tannase improved markedly.

关 键 词：单宁酶 固定化 戊二醛 交联 

分 类 号：TQ033[化学工程]