金枪鱼鱼骨胶原肽的制备及抗氧化活性研究  

Preparation and evaluation of an antioxidant peptide from collagen hydrolysate of skipjack tuna fishbone

作　　者：谭洪亮[1] 郁迪[1] 王斌[1] 胡发远[1] 马剑茵[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316022

出　　处：《水产学报》2014年第1期143-148,共6页Journal of Fisheries of China

基　　金："十二五"国家科技支撑计划(2012BAD29B06);浙江省重大科技专项(2011C02003);浙江省自然科学基金项目(Y2110636)

摘　　要：为制备金枪鱼鱼骨胶原肽,并对其抗氧化活性进行研究,利用酶解、超滤、凝胶色谱和反相高效液相色谱制备抗氧化胶原肽,采用氨基酸序列分析仪测定其氨基酸序列,利用质谱(ESIMS)确定其分子量,采用羟自由基、DPPH自由基、ABTS自由基和超氧阴离子自由基清除实验和脂质过氧化抑制实验对胶原肽抗氧化能力进行评价。结果显示,金枪鱼鱼骨胶原蛋白经胃蛋白酶和胰蛋白酶2步酶解和分离纯化得到1个十肽(TFCH-P2),经氨基酸序列分析和质谱(ESIMS)确定其氨基酸序列为Gly-Pro-Ala-Gly-Pro-Ala-Gly-Glu-Gln-Gly(GPAGPAGQEG),分子量为839.87 u([M+H]+840.68 u)。体外抗氧化实验结果表明,GPAGPAGQEG对羟自由基(EC500.18 mg/mL)、DPPH自由基(EC500.97 mg/mL)、ABTS自由基(EC500.52 mg/mL)和超氧阴离子自由基(EC500.38 mg/mL)具有良好的清除作用;GPAGPAGQEG亦显示出良好的脂质过氧化抑制作用。研究表明,胶原肽GPAGPAGQEG抗氧化活性良好,可以用于抗氧化相关的功能食品、药物或者食品添加剂。In the current research, acid soluble collagen （ ASC ） from skipjack tuna （ Katsuwonus pelamis） fishbone was successfully isolated with the yield of 2.47% （ dry weight basis）. The antioxidant hydrolysate of ASC（TFCH）was prepared through a two-stage in vitro digestion（3-h pepsin followed by 3-h trypsin） , and one new antioxidant peptide （TFCH-P2）was further isolated from TFCH using ultrafiltration, gel chromatography,and RP-HPLC. The amino acid sequence of TFCH-P2 was identified as Gly-Pro-Ala-Gly- Pro-Ala-Gly-Glu-Gln-Gly（GPAGPAGQEG） with molecular weight of 839.87 u （ [ M ＋ H ]^ ＋ 840.68 u）. TFCH-P2 exhibited good scavenging activity on DPPH radical, hydroxyl radical, ABTS radical and superoxide anion radical with ECs0 of 0.18,0. 97,0. 52 and 0.38 mg/mL, respectively. TFCH-P2 was also effectively against lipid peroxidation in a linoleic acid model system. The antioxidant activity of TFCH-P2 was due to the presence of hydrophobic amino acid residues within the peptide sequence. The result suggested that TFCH-P2 could be used as natural antioxidant in enhancing antioxidant properties of functional foods and in preventing oxidation reactions in food processing.

关 键 词：金枪鱼 鱼骨 多肽 抗氧化活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]