一株产低温蛋白酶菌株的筛选、鉴定及生长特性的研究  

Screening,identification and growth characteristics of a low-temperature proteinase producing strain

作　　者：张晓燕[1] 古丽娜孜[1] 库米拉[1] 陈卫林[1] 王小标[1] 杨静[1] 王雪薇[1] 韩艳鹏 武运[1] 

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《食品工业科技》2014年第5期145-148,152,共5页Science and Technology of Food Industry

基　　金：自治区科技支疆项目(201191238)

摘　　要：以新疆阿勒泰地区的冻土为原材料,采用平板分离法初筛,测蛋白酶活性法复筛,筛选出9株低温蛋白酶产生菌株,其中菌株A29-2蛋白酶活性最高,为31.88U/mL,且该菌在初始pH 7.0,NaCl质量分数为1.00%,22℃条件下繁殖量最大。通过形态学观察、生理生化鉴定及16S rDNA序列鉴定,鉴定为:该菌属于沙雷氏菌(Serratia sp.),该菌株所产的蛋白酶在4℃条件下保存48h,酶活力基本保持不变,表明该低温耐冷菌所产的蛋白酶为低温酶。Through the plate separation and the proteinase activity determination, nine low-temperature proteinase producing strains were obtained from the permafrost in the region of Altai,Xinjiang, in which the strain A29-2 had the highest proteinase activity, reaching to 3t.88U/mL.The growth of A29-2 reached the maximum level under the condition of initial pH？.0,mass fraction of NaCI 1.00% ,temperature 22℃.Based on the morphological observation, physiological and biochemical identification and 16S rDNA sequence analysis,the strain A29-2 was identified as Serratia sp..The activity of proteinase produced by A29-2 remained mostly unchanged after 48h of stored at 4℃. The results indicated that proteinase produced by cold-adapted strains was low-temperature enzyme.

关 键 词：低温蛋白酶 筛选鉴定 生长特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]