响应曲面法优化浙东白鹅皮胶原蛋白提取条件  

Optimization of Extraction Conditions for Collagen from Zhedong White Goose Skin by Response Surface Methodology

作　　者：丁琳[1] 张玉林[1] 杨媛[1] 赵紫微[1] 潘道东[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《食品科学》2014年第2期56-61,共6页Food Science

基　　金：国家水禽产业技术体系基金项目(CARS-43-17);宁波市创新团队项目(2012B82017);宁波市人事局人才基金项目(ZX2012000380);浙江省重大科技计划项目(2012C12016-1)

摘　　要：以浙东白鹅皮为对象,探讨酸种类、pH值、料液比及提取时间对胶原蛋白提取率的影响,先确定酸的种类,然后在单因素试验分析的基础上,采用响应曲面优化提取工艺。结果表明:最佳提取工艺为酸种类乳酸、pH 1.9、料液比1∶50(g/mL)、提取时间60 h。在此条件下,鹅皮胶原蛋白提取率达到30.12%,与理论预测值相比相对偏差为0.13%。经紫外光谱推断为典型的Ⅰ型胶原蛋白,红外光谱分析可得出其具有完整的三螺旋结构。The objective of this study was to optimize the acidic extraction of collagen from Zhedong white goose （a famous local breed of Chinese geese for meat production） skin. Acid type, pH, solid-to-liquid ratio and extraction time were considered in this study. Lactic acid was selected as optimal extraction solvent. One-factor-at-a-time method and response surface methodology were designed for the optimization of extraction conditions. The maximum extraction yield of collagen was experimentally observed as 30.12% under the optimized conditions： pH 1.9, solid-to-liquid ratio （g/mL） of 1：50 and extraction time of 60 h, showing a relative deviation of 0.13% compared with the predicted value. The sample obtained was identified as type I collagen by ultraviolet spectroscopy. Meanwhile, intact triple helix structure was also identified by infrared spectroscopy.

关 键 词：鹅皮 胶原蛋白 乳酸 响应曲面 

分 类 号：TS201.2[轻工技术与工程—食品科学]