有机体系中酶法催化合成甘油二酯工艺研究  

Lipase-catalyzed synthesis of diacylglycerol in organic solvent

作　　者：朱振雷[1] 操丽丽[1,2] 姜绍通[1,2] 潘丽军[1,2] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽省农产品精深加工重点实验室,安徽合肥230009

出　　处：《粮食与油脂》2014年第2期25-29,共5页Cereals & Oils

基　　金："十二五"国家科技支撑计划食品专用油脂加工关键技术和装备研究与示范(2011BAD02B04)

摘　　要：以大豆油和单硬脂酸甘油酯为原料,在有机溶剂体系中,采用固定脂肪酶催化转酯化合成甘油二酯。考察了不同有机溶剂、酶的种类、反应温度、反应时间、酶添加量以及底物摩尔比对甘油二酯得率影响。在单因素实验的基础上,通过响应面试验设计,确定最佳合成工艺条件为:固定脂肪酶Lipozyme RM IM作为催化酶,反应介质叔丁醇,反应温度52℃,时间6.70 h,脂肪酶添加量5.69%,底物摩尔比1.11∶1,此条件下,产物中甘油二酯的含量达到45.36%。通过二级分子蒸馏分离,甘油二酯含量达到92.31%。In this study,diacylglycerol（DAG）was synthesized by enzymatic transesterification in the organic solvent between soybean oil and monostearin. The effects of organic solvent,lipase, reaction temperature,reaction time,lipase dosage and substrate molar ratio on the content of DAG were investigated. Based on single factor experiment,the reaction conditions were optimized by response surface methodology as follows：tertiary butanol used as a solvent,immobilized Lipozyme RM IM used as a catalyst,reaction temperature of 52 ℃,reaction time of 6.70h,lipase dosage of 5.69%, subtract mole ratio of 1.11∶1. Under the optimal conditions,DAG content of the reaction production was 45.36%. After the purification by two-step distillation,content of DAG reached 92.31%.

关 键 词：甘油二酯 大豆油 单硬脂酸甘油酯 固定脂肪酶 

分 类 号：TS225.6[轻工技术与工程—粮食、油脂及植物蛋白工程]