亚甲基蓝对单增李斯特菌菌膜的光动力杀伤作用  

Antimicrobial Photodynamic Activity of Methylene Blue against Listeria monocytogenes Biofilms

作　　者：李红爱[1] 唐姝姝[1] 刘永强 邓曦[1] 唐书泽[1] 吴希阳[1] 陈振强[1] 

机构地区：[1]暨南大学理工学院,广东广州510632 [2]广州皇上皇集团有限公司,广东广州510240

出　　处：《食品科学》2014年第3期144-147,共4页Food Science

基　　金：广东省自然科学基金资助项目(S2012010008479);广东省突发公共事件应急技术研究中心专项([2011]733)

摘　　要：探讨单增李斯特菌(Listeria monocytogenes,LM)生物菌膜的生长特性及亚甲基蓝对LM生物菌膜光动力杀伤作用。通过结晶紫染色法判断与观察LM生物菌膜的形成并用酶标仪在595 nm波长处对其不同生长阶段的生物量进行测定,同时通过细菌平板菌落计数法研究亚甲基蓝对LM生物菌膜的光动力杀伤作用。结果表明:结晶紫染色法可用于定性判断与观察LM生物菌膜,且随着培养时间的延长,LM生物菌膜生物量不断增加,形成的网状结构越来越致密。当光敏剂质量浓度为10μg/mL的亚甲基蓝在光功密度为200 mW/cm2的可见光照射30 min时,即可使LM生物菌膜的失活率达到99.99%以上,其菌落数降低了4.08(lg(CFU/mL))。亚甲基蓝对LM生物菌膜的光动力灭活作用非常显著,其杀伤效果主要取决于光敏剂质量浓度和光照时间。The formation of Listeria monocytogenes（LM） biof ilms（BF） and its photodynamic inactivation by methylene blue（MB） were investigated. The LM BF formation process was identif ied by crystal violet staining, and the amount of biomass was tested with an enzyme-linked immunosorbent assay（ELISA） plate reader at 595 n m. The inactivation effect of MB on LM BF was verif ied by counting colony-forming units（CFU）. Res ults indicated that crystal violet staining could be used to visualize and quant ify LM BF. The amount of biomass increased and the formed network structure became more complicated as the incubation time was extended. Over 99.99% of LM BF was inactivated and colony-forming units were reduced by 4.08（lg（CFU/mL）） after illumination with visible light（power density 200 mW/cm2） for 30 min. LM BF were effectively inactivated by MB, and the eff icacy mainly dep ended on the concentration of photosesitizer and illumination time.

关 键 词：生物菌膜 单增李斯特菌 亚甲基蓝 光动力灭菌技术 

分 类 号：TS207.4[轻工技术与工程—食品科学]