大孔吸附树脂对草鱼肽的脱盐作用  

Desalination of Grass Carp Peptides with Macroporous Adsorption Resin

作　　者：林利美[1] 胡勤玲[1] 王申[1] 吴永宁[1,2] 宫智勇[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023 [2]国家食品安全风险评估中心,北京100021

出　　处：《食品科学》2014年第5期33-36,共4页Food Science

基　　金：国家高技术研究发展计划(863计划)项目(2010AA023003);武汉工业学院校研究生创新基金资助项目(2011cx013)

摘　　要：目的:研究DA201-C大孔吸附树脂对草鱼肽的脱盐作用。方法:对脱盐前后草鱼肽的灰分、肽含量、分子质量及氨基酸组成进行分析,采用脱盐率、血管紧张素转化酶(angiotensin I-converting enzyme,ACE)抑制活性、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH)自由基清除率和铁还原抗氧化能力(ferric reducing antioxidant potential,FRAP)为指标考察其脱盐效果。结果:草鱼肽的脱盐率为82.02%,脱盐处理后,肽片段和疏水氨基酸得到有效富集;ACE抑制活性显著增大,半抑制质量浓度(IC50)由0.36 mg/mL变为0.23 mg/mL;抗氧化性增强,其IC50由5.59 mg/mL变为4.48 mg/mL;总抗氧化值由1.018增大到1.261。结论:采用DA201-C大孔吸附树脂对草鱼肽脱盐是一种简便有效的处理方法。Objective： To investigate the desalination of grass carp peptides （GCP） with macroporous adsorption resin DA201-C. Methods： Ash and peptide contents, molecular weight and amino acid composition of GCP were analyzed before and after desalination. Desalination efficiency, angiotensin I-converting enzyme （ACE） inhibitory activity, 2,2-diphenyl-1- picrylhydrazyl （DPPH） radical scavenging activity and ferric reducing antioxidant potential （FRAP） were used to evaluate how well GCP was desalinated. The results showed that the desalination efficiency of GCP was 82.02%. The peptide fragments and hydrophobic amino acids were enriched in desalinated GCP. Meanwhile, the ACE inhibitory activity was significantly higher than that of native GCP, showing a decrease in IC50 from 0.36 to 0.23 mg/mL, and the antioxidant activity was also increased showing an IC50 of 4.48 mg/mL compared to 5.59 mg/mL for native GCP. The desalination resulted in an increase in the FRAP value of GCP from 1.018 to 1.261. Conclusion： The use of macroporous adsorption resin DA201-C provides a simple and effective method to desalinate GPC.

关 键 词：大孔吸附树脂 草鱼肽 脱盐 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]