罗非鱼肉蛋白酶解液的制备及体内外抗氧化活性研究  

Preparation of Tilapia(Oreochromis niloticus) Protein Hydrolysate and Analysis of Its Antioxidant Activity in vitro and in vivo

作　　者：王强[1] 李贵节[1] 赵欣[1] 任彦荣[1] 

机构地区：[1]重庆第二师范学院生物与化学工程系,重庆400067

出　　处：《食品科学》2014年第5期199-203,共5页Food Science

基　　金：重庆市教委科学技术研究项目(KJ121504);重庆高校创新团队建设计划资助项目(KJTD201325)

摘　　要：采用木瓜蛋白酶酶解罗非鱼肉蛋白,通过测定不同剂量罗非鱼肉蛋白酶解液的还原力,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH)自由基、·OH和O2-·清除率及对D-半乳糖致衰老模型小鼠的抗氧化作用,评价罗非鱼肉蛋白酶解液的体内外抗氧化特性。结果表明:罗非鱼肉蛋白酶解液具有较高的还原力,清除DPPH自由基、·OH和O2-·的半数有效质量浓度分别为1.57、1.68 mg/mL和2.76 mg/mL;罗非鱼肉蛋白酶解液能显著提高小鼠血清和肝脏组织中超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力和总抗氧化能力(P<0.05),同时使血清和肝脏组织中的丙二醛含量显著降低(P<0.05)。罗非鱼肉蛋白酶解液有较强的体内外抗氧化活性。Tilapia （Oreochromis niloticus） protein hydrolysate was prepared with papain. Its antioxidant activities at different doses were evaluated in vitro and in vivo using reducing power, 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-l-（2,4,6-trinitrophenyl）hydrazyl （DPPH）, hydroxyl and superoxide anion radical scavenging assays and D-galactose-induced mouse aging model. The reducing power of the hydrolysate was high. The ECs0 values in the DPPH radical, hydroxyl and superoxide anion radical scavenging assays were 1.57, 1.68 and 2.76 mg/mL, respectively. The hydrolysate could significantly increase the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） and total antioxidative capacity （T-AOC） in the serum and liver of aging mice （P 〈 0.05）. Furthermore, the content of malondialdehyde （MDA） in serum and liver displayed a reducing trend （P 〈 0.05）. In conclusion, the tilapia protein hydrolysate has a good antioxidant effect in vitro and vivo as expected.

关 键 词：罗非鱼肉 酶解液 体外抗氧化活性 D-半乳糖衰老模型小鼠 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]