响应面优化超声波提取猕猴桃根熊果酸工艺  

Optimization of Ultrasonic-Assisted Extraction of Ursolic Acid from Roots of Actinidia chinensis Using Response Surface Analysis

作　　者：杨艳[1] 任亚梅[1] 马婷[1] 周九庆 查晶[1] 张哲学[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2014年第4期44-49,共6页Food Science

基　　金：西北农林科技大学科技创新与成果转化项目(Z222021313)

摘　　要：为优化猕猴桃根熊果酸的超声波提取最佳工艺,在单因素试验基础上,选择乙醇体积分数、液料比、提取时间、提取温度为自变量,熊果酸得率为响应值,利用中心组合试验法和响应面分析法,研究各自变量交互作用及其对熊果酸得率的影响,模拟得到二次多项式回归方程的预测模型。结果表明:当超声功率225 W、超声频率40 kHz时,超声波提取熊果酸工艺最佳条件为乙醇体积分数73.8%、液料比7.80∶1(mL/g)、提取时间23.2 min、提取温度79.1℃。此提取条件下,熊果酸得率达到2.014%,与模型预测值之间具有较好的拟合性,且提取液中熊果酸的纯度为49.7%。In this study, the conditions for ultrasonic-assisted extraction of ursolic acid （UA） from the roots of Actinidia chinensis were optimized by response surface methodology. On the basis of single-factor experiments, a mathematical regression model was established indicating the effect of ethanol concentration, solvent/solid ratio, extraction time and temperature on the extraction yield of UA by response surface central composite design. Under the conditions of 225 W and 40 kHz, the optimum extraction conditions determined were as follows： 73.8% ethanol concentration, 7.80：1 solvent/solid ratio （mL/g）, 23.2 min and 79.1℃. Under these conditions, the experimental extraction yield of UA was 2.014%, which is well matched with the predicted value, and the purity of UA in the extract was 49.7%.

关 键 词：猕猴桃根 熊果酸 超声波提取 响应面分析 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]