山楂黄酮超声波辅助提取工艺参数优化研究  

Optimization of extracting technique of flavonoids by ultrasound assisted extraction method from hawthorn

作　　者：孙协军[1] 李秀霞[1] 刘雪飞[1] 励建荣[1] 吕艳芳[1] 李娇[1] 王珍[1] 田鑫[1] 

机构地区：[1]渤海大学化学化工与食品安全学院辽宁省食品安全重点实验室辽宁省高校重大科技平台"食品贮藏加工及质量安全控制工程技术研究中心",辽宁锦州121013

出　　处：《食品工业科技》2014年第9期203-207,共5页Science and Technology of Food Industry

基　　金：“十二五”国家科技支撑计划(2012BAD29B06);辽宁省食品安全重点实验室开放课题(LNSAKF2011015)

摘　　要：为了研究山楂黄酮的组成和含量,本实验以乙腈和0.4%磷酸水溶液为流动相,建立了山楂芦丁、金丝桃苷和槲皮素的HPLC检测方法,采用外标法定量,以3种黄酮总得率为响应值,对山楂黄酮的超声波辅助提取工艺参数进行了优化研究,并建立了山楂黄酮超声波辅助提取的数学模型。结果表明,各提取因素影响的主次顺序为:液固比>乙醇体积分数>提取时间>超声波功率;通过对提取工艺的研究,确定了超声波辅助提取山楂黄酮提取的最佳工艺参数为:乙醇体积分数90%,液固比25.5mL/g,超声波功率600W,提取时间为24.9min,该工艺条件下的山楂黄酮得率为0.0363%。芦丁、槲皮素和金丝桃苷是山楂中主要的黄酮类物质,而超声波辅助提取可以有效地从山楂中提取得到这三种黄酮。In order to investigate the composition and content of hawthorn flavonoids, a gradient high performance liquid chromatography （HPLC）was developed for the detection of rutin, hyperoside and quercetin from hawthorn.Acetonitrile and 0.4% phosphate solvent was used as mobile phase, external standard method was adopted for quantification of three flavonoid yield, extraction technique of the three flavonoid yield was optimized and the mathematical model was established.The results showed that the sequence of factors was as follows .liquid to solid ratio 〉 volume fraction of ethanol 〉 extracting time 〉 ultrasonic power, the optimal condition for ultrasound assisted extraction condition was that volume fraction of ethanol 90%, liquid to solid ratio 25.SmL/g, ultrasonic power 600W,extracting time 24.groin, under the optimal extraction conditions, the flavonoid yield was 0.0363%.1n this test, it was found that rutin, hperoside ande quercetin were the main flavonoids in hawthorn,which could be extract efficiently by ultrasound assisted method.

关 键 词：山楂 芦丁 金丝桃苷 槲皮素 超声波辅助提取 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]