响应面法优化可口革囊星虫抗氧化多糖提取工艺研究  

Optimization of extraction process by response surface methodology of anti-oxidant polysaccharides from Phasolosma esculenta

作　　者：孔帅[1] 刘连亮[1] 陆新江[1] 史雨红[1] 陈炯[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《食品工业科技》2014年第11期233-237,共5页Science and Technology of Food Industry

基　　金：浙江省海洋生物技术产业科技创新团队项目(2012R10029-05);浙江省教育厅科研项目资助(Y201430696);宁波大学校科研基金项目(013-E00841144702)

摘　　要：本研究利用响应面法优化可口革囊星虫抗氧化多糖的提取工艺。在单因素实验的基础上,选取提取时间、提取温度、加酶量三个因素,根据Box-Benhnken中心组合实验设计原理,确定提取工艺的最佳条件。结果表明:可口革囊星虫多糖的最佳提取工艺条件为提取温度65℃,提取时间7h,加酶量10000U/g。在此条件下,星虫粗多糖的得率为0.879%±0.001%,DPPH·清除率为72.5%。Response surface methodology was employed to optimize conditions for the extraction of anti-oxidant polysaccharides from Phasolosma esculenta.Based on the single- factor tests, extraction time, extraction temperature and enzyme amount were selected out from extraction conditions for the three-level Box-Behnken experimental design.The results showed that optimal extraction process parameters were 65℃,7h and 10000U/g. Under these conditions,the experimental yields of polysaccharides was 0.8？9% ± 0.001% ,while DPPH. scavenging activity was 72.5 %.

关 键 词：可口革囊星虫 粗多糖 响应面法 酶解 

分 类 号：TS201.2[轻工技术与工程—食品科学]