宇佐美曲霉Y-26纤维素酶的纯化及酶学性质  

Purification and Properties of Cellulose Activity from A.usamii

作　　者：潘锋[1] 杨树林[1] 史小丽[1] 包晓峰[1] 

机构地区：[1]南京理工大学化工学院,南京210094

出　　处：《南京理工大学学报》2001年第4期424-427,共4页Journal of Nanjing University of Science and Technology

基　　金：南京市科学技术委员会资助项目 (宁科 (1 999) 992 2 1 )

摘　　要：从宇佐美曲霉 (A .usamii)固态发酵物中提取纤维素酶。粗酶液经硫酸铵盐析除去大量杂蛋白 ,然后通过 2次SephadexG - 2 0 0柱层析纤维素酶中CMC酶 ,提纯倍数可达 5 72。酶学研究表明 ,纤维素酶中CMC酶最适作用温度为 6 0℃ ,最适作用pH为 4.0 ,30～ 6 0℃区间酶活力较稳定 ,在pH为 3 .0～ 5 .0范围内 ,5 0℃保温 30min ,能保持 90 %的酶活力。红外光谱分析表明 ,纤维素酶在O—H ,N—H ,C—H ,CO有吸收带。CMC酶的Km、vmax 值分别为 0 10 g/ml、0 40mg/(ml·min)。The cellulose was extracted from solid state leavening of A usamii. The crude product was purified by (NH 4) 2SO 4 and chromatographed by Sephadex G-200 column gel. The CMC component of cellulose is 6 times of raw products. The study on the enzymology of CMC enzyme shows that the best reaction temperature is 60 ℃, pH is 4.0, and enzyme is relatively stable at 30～60 ℃. The 90 % activity can be held in pH is 3.0～5.0 for 30 min at 50 ℃. There are IR spectrum absorption bands of O-H,N-H,C-H,CO. The values of CMC (K m ,v max ) were 0.10 g/m and 0.40 mg/(ml·min) respectively.

关 键 词：纤维素酶 纯化 酶学 宇佐美曲霉 固态发酵法 

分 类 号：Q556.2[生物学—生物化学]