毛细管区带电泳法分离发酵液中的木糖和木糖醇  

Separation of Xylose and Xylitol in Fermentation Liquid by Capillary Zone Electrophoresis

作　　者：陈冠华[1] 张利平[2] 田益玲[1] 王秀敏[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北大学生命科学学院,河北保定071002

出　　处：《色谱》2001年第6期549-551,共3页Chinese Journal of Chromatography

基　　金：河北省教育厅自然科学基金资助项目 (项目号 :931315 )

摘　　要：建立了利用毛细管区带电泳分离发酵液中木糖和木糖醇的新方法。研究表明 :采用硼砂缓冲溶液时 ,木糖和木糖醇的分离度随硼砂浓度的增高而加大 ,在室温下硼砂最高浓度为 130mmol/L ;分离度还与溶液的 pH有关 ,在pH 9 5 5处分离度有最大值 ;缓冲液中十六烷基三甲基溴化铵的浓度为 4× 10 -6mmol/L～ 8× 10 -4 mmol/L时对分离度无显著影响 ;在优化的分离条件下 ,木糖和木糖醇可在 6min内基线分离。测定了发酵过程中样品各组分的含量和加标回收率 ,5次测定木糖的相对标准偏差 (RSD)为 1 42 %～ 3 11% ,回收率为 96 0 %～ 10 8 0 % ;5次测定木糖醇的RSD为 0 6 2 %～ 1 32 % ,回收率为 94 0 %～ 10 9 0 %Xylitol may be produced with microbial fermentation technology when xylose is used as the raw material. It is important in scientific research and production fields concerned with xylose and xylitol to develop the method of separation and detection. The anionic complexes with strong UV absorption at 195 nm will form by dissolving xylose and xylitol in borax solution. They may be separated with borax buffer by use of capillary zone electrophoresis. It was shown that the resolution between xylose and xylitol gradually increased with the increase of borax concentration, but its maximum concentration was 130 mmol/L at room temperature. The resolution depended on the pH of running buffer with a maximum at pH 9 55. The resolution was independent of cetyltrimethylammonium bromide when its concentration was between 4×10 -6 mmol/L 8×10 -4 mmol/L. So the optimum conditions were as follows:borax concentration of 130 mmol/L, pH 9 55 and cetyltrimethylammonium bromide concentration of 5×10 -5 mmol/L in running buffer; separating voltage of -12 kV; column temperature of 25 ℃. Xylose and xylitol could be separated on base line in 6 min under these conditions. Samples from fermentation process and the recoveries of spiked samples were determined. The relative standard deviations of the results were between 1.42% 3.11% for xylose, and 0.62% 1.32% for xylitol. The recoveries were between 96.0% 108 0% for xylose and 94.0% 109 0% for xylitol.

关 键 词：毛细管区带电泳 木糖 木糖醇 发酵液 分离 微生物发酵 

分 类 号：TQ923[轻工技术与工程—发酵工程]