人era真核表达载体的构建  

Construction of human era eukaryotic expression vector with HA tag

作　　者：纪宗玲[1] 陈苏民[1] 刘继中[2] 吴元明[1] 路凡[1] 柴玉波[1] 李毅[1] 张俊杰[1] 

机构地区：[1]第四军医大学生物化学与分子生物学教研室 [2]第四军医大学西京医院全军骨科研究所,西安710032

出　　处：《生物技术通讯》2002年第3期170-172,共3页Letters in Biotechnology

基　　金：国家自然科学基金(39870380;30000091);全军医药卫生科研基金(98M108)

摘　　要：Era是一个独特的含有RNA结合KH结构域的G蛋白亚家族,哺乳类era新近被克隆,目前还没有关于其功能的研究报道。构建了带流感病毒血凝素9肽表位标签(HAtag)的野生型人era基因的真核表达载体,瞬时转染体外培养细胞CHO,Westernblot鉴定表明目的基因得到表达。为深入探讨人era基因的功能奠定了坚实的基础。Era is a unique G protein subfamily with RNA-binding KH domain,mammalian era was cloned recently and there is no report on its function yet.In this report eukaryotic expressive vector of human era with HA tag was constructed and than was transfected into CHO cell line using lipid transfection.Expression was detected by western blot with anti-HA monoclonal antibody.These results may shed light on the further study of the function of human era.

关 键 词：人ERA 真核表达载体 构建 基因表达 CHO细胞 

分 类 号：Q782[生物学—分子生物学] R394.8[医药卫生—医学遗传学]