椰果表面混菌生物膜培养条件优化  

Optimization of culture condition of mixed-species biofilm on coconut

作　　者：浦明珠 李伟[1] 陈晓红[1] 姜梅[1] 芮昕[1] 董明盛[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品工业科技》2014年第15期159-162,共4页Science and Technology of Food Industry

基　　金：国家"863"计划项目(2011AA100903;2013BAD18B01-4);国家自然科学青年基金项目(31371807;31201422);江苏省自然科学基金项目(BK2011651);教育部博士点基金项目(新教师类)(20110097120028);农业科技成果转化资金项目(2012GB23600639);江苏高校优势学科建设工程项目(PAPD)

摘　　要：为了探明椰果表面混菌生物膜的最优培养条件,采用单因素和正交实验法对保加利亚乳杆菌(Lactobacillus bulgaricus)和嗜热链球菌(Streptococcus thermophilus)在椰果表面形成的混菌生物膜的培养条件进行优化。分别选取培养时间、培养温度、固液比、接种量四个因素。研究结果表明,四个因素对椰果表面形成的混菌生物膜上活菌数的影响效果为:培养温度>培养时间>固液比>接种量。椰果表面混菌生物膜培养条件的优化结果为:培养时间2.5d,培养温度为37℃,椰果与MRS液体培养基组成比例为1∶30(g/mL),接种量为2%。在此培养条件下培养的混菌生物膜,其活菌数达到最大值,为5.79×108CFU/cm2。In order to explore the optimized culture condition of mixed-species biofilm on coconut, the culture of mixed-species biofilm on coconut formed by Lactobacillus bulgaricus and Streptococcus thermophilus was optimized by single factor and orthogonal test. Four factors （time, temperature, solid- liquid ratio, inoculation amount） were chosen.Results showed the effect of these factors on the viable count of mixed-species biofilm on coconut：temperature 〉 time 〉 solid-liquid ratio 〉 inoculation amount.The optimized culture condition of mixedspecies biofilm on coconut was ;time 2.5d ,temperature 37℃,solid-liquid ratio 1：30（ g/mL）, inoculation amount 2%. Under such condition, the viable count of mixed- species biofilm on coconut reached the maximum of 5.79 × 10^ 8 CFU/cm2.

关 键 词：乳酸菌 生物膜 椰果 培养条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]