高效液相色谱法测定市售泡凤爪中的生物胺  

Determination of biogenic amines in commercial bubble chicken legs by HPLC

作　　者：王瑜[1] 瞿凤梅 丁晓雯[1] 

机构地区：[1]西南大学食品科学学院,重庆市农产品加工重点实验室,重庆400715

出　　处：《食品工业科技》2014年第15期315-319,共5页Science and Technology of Food Industry

基　　金：国家级大学生创新创业训练计划(201310635010)

摘　　要：采用丹磺酰氯为柱前衍生，建立高效液相色谱法同时测定市售泡凤爪中色胺、β-苯乙胺、腐胺、尸胺、组胺、酪胺、亚精胺、精胺8种生物胺含量的方法。色谱条件：C18色谱柱分离，以水和乙腈为流动相梯度洗脱，流速0．8mL／min，紫外检测器，检测波长：254nm。在此条件下，8种生物胺在23min内实现了良好的分离，该方法标准曲线相关系数均在0．998以上，精密度在可接收范围内（RSD〈5％），8种生物胺的平均回收率在90．41％～99．66％之间。结果表明该方法灵敏度高，精密度和重复性好，可快捷、准确地对市售泡凤爪中的生物胺进行检测。检测出生物胺总量范围在72．42～409．67mg／kg，其中，色胺、组胺、腐胺、尸胺、亚精胺的检出率为100％，其平均含量分别为47．53、26．20、35．65、12．94、8．15mg/kg。The samples in experiment derivatized with dansyl chloride.A high performance liquid chromatographic （HPLC） method was developed for the simultaneous determination of tryptamine, β-phenylethylamine, putrescine, cadaverine, histamine, tyraminespermi - dine, and spermine in commercial bubble chicken legs. The chromatographic sep-aration was carried out on a C18 column.The gradient elution program with a mixture of ultrapure water and acetonitrile at the flow rate 0.8mL/min was used under UV detector with a wavelength of 254nm.Under this experimental condition,8 amines had a good resolution in 23min and well linearity（P〉 0.998）in detection range.The relative standard deviation（RSD） of precision was lower than 5% respectively.The average recoveries ranged from 90.41% to 99.66% for all amines.The results showed that,with the merits of high sensitivity, precision and well repeatability, the HPLC method was convenient, rapid and reliable for biogenic amines detection. The contents of biogenic amines were ranging between 72.42 and 409.67mg/kg.Among them, the detection of tryptamine, cadaverine, histamine, putrescine and spermidine was 100%, the average contents were 47.53,26.20,35.65, 1 2.94,8. 15 m g/kg.

关 键 词：高效液相色谱 生物胺 泡凤爪 

分 类 号：TS207.3[轻工技术与工程—食品科学]