中低压C_(18)柱层析分离儿茶素单体的研究  

Study on purification of catechins by C_(18) column chromatography

作　　者：赵文芳[1] 黄亚辉[1,2] 

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]华南园艺作物种质创新与利用广东省普通高校重点实验室,广东广州510642

出　　处：《食品工业科技》2014年第17期97-101,106,共6页Science and Technology of Food Industry

基　　金：广东省重点攻关项目(2009B020201013)

摘　　要：本文采用中低压柱层析制备色谱仪,研究了AB-8大孔吸附树脂、C18、聚酰胺树脂、硅胶4种不同柱层析吸附材料对茶多酚的分离效果,最终选择C18作为最佳层析材料,考察了不同的茶多酚进样量、流速对儿茶素分离效率的影响。结果表明以C18作为柱层析材料,甲醇水溶液进行梯度洗脱,可得到EGCG、GCG、ECG、CG四种儿茶素,其最高总得率为68.44%,且确定最佳工艺条件:350mg茶多酚进样量在20mL/min流速下进行甲醇水溶液梯度洗脱,可获到EGCG,GCG,ECG,CG四种儿茶素,其得率分别为36.19%±0.11%、7.42%±0.14%、12.93%±0.18%、2.40%±0.22%,而其纯度分别为88.85%±0.17%、83.74%±0.09%、96.01%±0.13%、70.67%±0.11%。该工艺操作简单,纯化分离效果较好;可为进行规模化、工业化生产提供参考。To obtain high-purity catechin monomers,the separation effect of AB-8 macroporous absorption resin, polyamide resin,silica gel,C18 by preparative medium pressure liquid chromatography（ MPLC）was investigated. C18 was chosen as the best filling materials to separate catechins components in condition of the different injection volume of tea polyphenols and different flow rates.The optimum technological conditions was as followed：C18 as packing material, methanol/water as eluting solvent, injection volume 350mg, flow rate 20mL/min. EGCG, GCG, ECG,CG were achieved with their yield of 36.19% ±0.11% ,7.42%±0.14% ,12.93% ±0.18% ,2.40% ±0.22% and purities were 88.85% ±0.12% ,83.74% ±0.09% ,96.01% ±0.13% ,70.67% ±0.11% ,respectively.The total recovery of all catechins was 68.44%. With easy operation and high yield, this process could be helpful to the efficient preparation of catechin monomers from tea polyphenols and met industrialized production.

关 键 词：茶多酚 儿茶素 柱层析 纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学]