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作 者:王菲[1,2] 佘星星[1] 孙冰洁[1] 杜嘉琛 张家玮[1] 任迪峰[1] 鲁军[2]
机构地区:[1]北京林业大学生物科学与技术学院北京市林业食品加工与安全重点实验室,北京100083 [2]中国食品发酵工业研究院北京市蛋白功能肽工程技术研究中心,北京100015
出 处:《食品工业科技》2014年第19期68-72,共5页Science and Technology of Food Industry
基 金:教育部新世纪优秀人才支持计划(NCET-11-0587);国家自然科学基金(31201339);国家林业公益性行业科研专项资金(201304805);国家高技术研究发展计划(863计划)项目(2013AA102205);十二五国家科技支撑计划(2012BAD33B04);北京市科委领军人才项目(Z131110000513026)支持
摘 要:以螺旋藻粉为原料,采用溶剂萃取法提取不饱和脂肪酸γ-亚麻酸,研究了提取溶剂、提取料溶比、提取温度、提取时间等工艺条件对提取得率的影响,并优化提取条件。此外对其体外清除自由基、抗脂质过氧化能力及对H2O2氧化损伤人正常肝细胞HL-7702保护能力进行研究。结果表明:乙醇为溶剂、料溶比1∶12(w/v)、提取温度70℃,提取时间90min时能够得到最高的GLA产量(8.3±0.17)g·kg-1(GLA/干量),温度对提取率影响最大。GLA提取物显示出了较强的脂质抗氧化能力,10mg·L-1GLA提取物的抑制效果优于100mg·L-1VC,但其清除自由基能力较低,表明脂质环境能够促进其抗氧化效果,且螺旋藻GLA提取物具有保护H2O2所致氧化损伤HL-7702细胞的效果。Polyunsaturated acid GLA was extracted from Spirulina platensis, and the effects of different solvent, solid-liquid ratio,reaction temperature and reaction time on GLA yield and optimal process were also discussed. Besides,free radical scavenging activity, antioxidant capacity of GLA extract and protective effect on H2O2- induced HL-7702 cells were investigated, respectively.The results showed that the ethanol extraction at 70℃ with a sample-solvent ratio (w/v) of 1 : 12 for 90 min achieved the highest GLA yield of (8.3 ± 0.17 ) g · kg -1 ( GLA/dry biomass).The reaction temperature was the most significant factor among others. GLA extract exhibited strong inhibition effect on lipid peroxidation,of which the inhibitory effect of 10mg· L-1 GLA extract was even higher than that of 100mg· L-1 Vitamin C,while tittle activity was detected in the free radical scavenging activity,indicating that lipid environment might facilitate its antilipid peroxidation function.Moreover, the GLA extract exhibited protection effects on HL-7702 cells from oxidative damage induced by H2O2.
分 类 号:TS201.4[轻工技术与工程—食品科学]
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