乙醇传感器法测定葡萄酒和黄酒中乙醇的含量  

Determination of alcohol in grape wine and rice wine by ethanol biosensor

作　　者：赵洪庆[1,2] 高红波[2,3] 钟其顶[2,3] 孟镇[2,3] 陈楠楠[2,3] 熊正河[2,3] 崔波[1] 

机构地区：[1]齐鲁工业大学食品与生物工程学院,山东济南250353 [2]中国食品发酵工业研究院,北京100015 [3]全国食品发酵标准化中心,北京100015

出　　处：《食品工业科技》2014年第19期293-296,共4页Science and Technology of Food Industry

基　　金：质检公益性行业科研专项(201210016);科技部支撑计划项目(2012BAK17B11);科技部国际合作项目(2011DFA33270);质检公益项目(200910220)

摘　　要：建立了生物传感器法测定葡萄酒和黄酒中乙醇含量的分析方法。结果表明：乙醇含量在2-80μL／100mL浓度范围内线性关系良好，相关系数为0．9997；加标回收率（n=3）在99．22％～101．59％之间；精密度和重复性（n=4）的RSD％低于1％。与国标酒精计法测定结果对比，两种方法无显著差异。该法具有无需样品前处理、特异性好、灵敏度高、测定速度快等优点，适用于企业对发酵过程控制和产品的质量检测。A method was developed for measurement of alcohol content in grape wine and rice wine by ethanol biosensor.A suitable linear relationship was obtained between the concentrations of alcohol（2-80μL/]00mL）and the correlation coefficient was 0.9997; the recovery ratio （ n = 3 ） were between 99.22% - 101.59%; the RSD% of precision and repeatability （n = 4）were blow 1%. Alcohol content in samples evaluated by this method was compared with those evaluated by alcohol meter method, and there was no significant difference of the two methods.This method is a simple, specific,sensitive, rapid for determination of alcohol in wines,suitable for winery to control or monitor the quality of finished wine and brewing wine.

关 键 词：生物传感器 葡萄酒 黄酒 乙醇 

分 类 号：TS207[轻工技术与工程—食品科学]