高效液相色谱-紫外检测法快速测定小麦面粉中偶氮甲酰胺  

Rapid determination of azodicarbonamide in wheat flour by HPLC-UV

作　　者：张桂芳[1] 张晓瑜[1] 宫月华[1] 孙兆鹏[1] 刘泽静[1] 韩文清[1] 

机构地区：[1]烟台市疾病预防控制中心,山东烟台264003

出　　处：《中国食品卫生杂志》2014年第5期473-475,共3页Chinese Journal of Food Hygiene

摘　　要：目的建立小麦粉中偶氮甲酰胺的高效液相色谱快速检测法。方法样品经二甲亚砜-丙酮混合溶剂超声振荡提取,采用Hypersil-C18色谱柱（250 mm×4.6 mm,5μm）,流动相为10 mmol/L H2SO4水溶液,流速为1 ml/min,检测波长为275 nm,进样量20μl,二极管阵列检测器定量。结果偶氮甲酰胺在0.01～1.00 mg/ml范围内呈良好线性关系,相关系数r=0.999 2,RSD〈4.3%,平均回收率为95.9%;山东地区85份样品偶氮甲酰胺含量检测结果均小于检出限,符合现行限量标准。结论本方法准确可靠,方便快捷,灵敏度高,适合于大批量小麦粉中偶氮甲酰胺的检测。Objective To establish a method for the determination of azodicarbonamide （ADA） in wheat flour by HPLC. Methods The samples were extracted by acetone-dimethyl sulfoxide mixture with ultrasonic oscillations. The ADA separation was carried out on a Hypersil-Cl8 column （250 mm×4.6 mm, 5 μm） with 10 mmol/L H2SO4 solution as mobile phase at a flow rate of 1 m]/min, and detected at 275 nm with a 20 μl injection volume. Results The linear range for ADA was 0.01-1.00mg/m1, and the correlation coefficient was 0. 999 2. The average recovery was 95.9% （RSD 〈 4.3% ）. The ADA content of 45 samples from Shandong province were lower than the detection limit, which were in line with the current standards. Conclusion The method was proved to be simple and accurate with good repeatability and high sensitivity, which could apply to the determination of ADA in bulk wheat flour.

关 键 词：面粉 偶氮甲酰胺 高效液相色谱 快速测定 面粉处理剂 食品添加剂 食品安全 

分 类 号：O657.72[理学—分析化学] R155[理学—化学]