紫外与亚硝基胍复合诱变选育高产多糖罗耳阿太菌  

Screening of high-yield polysaccharide Athelia rolfsii by UV and NTG

作　　者：李鸿梅[1] 苗琇岩[1] 魏明[1] 赵慧[1] 闵伟红[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品工业科技》2014年第20期244-247,262,共5页Science and Technology of Food Industry

基　　金：吉林省科技发展计划项目(20126037)

摘　　要：以实验室保存的罗耳阿太菌(Athelia rolfsii)为出发菌株,分别采用紫外线(UV)和亚硝基胍(NTG)诱变方法选育高产多糖菌株。与出发菌株相比,紫外诱变菌株多糖产量增加了10.70%,亚硝基胍诱变菌株多糖产量增加了15.78%。通过响应面设计紫外线和亚硝基胍复合诱变,得到正向突变株,其中X2突变株遗传性状稳定,多糖产量达19.868g/L,相比出发菌株增长了23.85%。High-yield polysaccharide Athelia Rolfsii from original Athelia Rolfsii preserved in laboratory was screened by UV and NTG. The data showed that the increasing rate of polysaccharide production was 10.70% from a strain by UV mutagenesis and 15.78% from a strain by NTG mutagenesis. X2 with stable polysaccharide production was obtainded by NTG combined with UV. The polysaccharide production from X2 was 19.868g/L. The increasinQ rate of Dolvsaccharide production from X2 was 23.85%.

关 键 词：罗耳阿太菌 亚硝基胍与紫外复合诱变 多糖产量 

分 类 号：TS24[轻工技术与工程—制糖工程]