毛细管电泳法同时测定饮料中的阿斯巴甜、糖精钠和安赛蜜  

Simultaneous determination of aspartame, sodium saccharin and acesulfame in drinks by capillary electrophoresis

作　　者：张静[1] 杨鸿斌[2] 谢娟[1] 卢丹[1] 陈璐莹[1] 刘亚攀[1] 孙成均[1,3] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]巴中市疾病预防控制中心,四川巴中636600 [3]四川省食品安全监测与风险评估重点实验室,四川成都610041

出　　处：《现代预防医学》2014年第20期3768-3770,共3页Modern Preventive Medicine

摘　　要：目的建立饮料中阿斯巴甜、糖精钠和安赛蜜的毛细管电泳-紫外检测法。方法样品经超声脱气,12 000r/min离心5 min后,取上清液直接进样分析。以熔融石英毛细管（50 cm×50μm,有效长度41 cm,未涂层）为分离柱;20 mmol/L硼砂溶液（pH=9.3,15%乙腈）为运行缓冲液,进样时间10 s,进样高度20 cm,分离电压15 kV,在210 nm的波长下,阿斯巴甜、糖精钠和安赛蜜在12 min内得以基线分离。结果阿斯巴甜、糖精钠、安赛蜜在5.0-200 mg/L范围内,标准曲线的线性相关系数分别为0.9997,0.9996和0.9997。其检出限分别为0.37μg/ml,0.12μg/ml和0.26μg/ml,峰面积相对标准差分别为4.0%,1.6%和4.1%。方法平均加标回收率分别为92.1%-111%,88.3%-111%和83.3%-115%。结论该方法快速灵敏,操作简单,适合于饮料中该3种甜味剂的同时检测。Objective The purpose of this study was to establish a methodology for simultaneous determination of three sweeteners including aspartame, sodium saccharin and acesulfame in drinks by capillary electrophoresis with UV detection. Methods The three sweeteners in drinks were directly analyzed after centrifuged at 12000 rpm. Regarding the separation conditions, separation column was a bare fused-silica capillary （50 mm i.d., 50 em of total length, and 41 em elective length） and the running buffer was 20 mmol/L sodium tetraborate in 15% acetonitrile （pH=9.3）. Under the separation voltage of 15 kV, gravity sampling time was 10 s with gravity sampling height of 20 em, and the detection wavelength was 210 nm. Aspartame, sodium saccharin and acesulfame were well separated within 12 min. Results There were good linear relationships in the range of 5.0-200 mg/L, with the linear correlation coefficients of 0.9997, 0.9996 and 0.9997, respectively. The detection limits were 0.37 μg/ml, 0.12μg/ml and 0.26μg/ml, respectively. The relative standard deviations were 4.0%, 1.6% and 4.1%, respectively. Spiked recoveries of the methodology were 92.1%-111%, 88.3%-111% and 83.3-115%, respectively. Conclusion The methodology is rapid, sensitive, simple and it is suitable for simuhaneous determination of the three sweeteners in drinks.

关 键 词：高效毛细管电泳法 阿斯巴甜 糖精钠 安赛蜜 饮料 

分 类 号：R15[医药卫生—营养与食品卫生学]