酵母源金属硫蛋白体外清除自由基及抑菌活性的研究  

Study on scavenging free radical and antibacterial activities in vitro of metallothioneins from yeast

作　　者：徐炳政[1] 王颖[1,2] 张东杰[1] 易伟民[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《食品工业科技》2014年第21期111-114,共4页Science and Technology of Food Industry

基　　金：省博士后资助项目(LBH-Z13169);省教育厅新世纪人才项目(2014-2016)

摘　　要：目的:比较两种异构体形式酵母源金属硫蛋白与动物源金属硫蛋白体外清除自由基及抑菌活性。方法:以VC为对照,分光光度法测定金属硫蛋白对自由基清除率,以金黄色葡萄球菌、大肠杆菌和李斯特氏菌为指示菌,研究金属硫蛋白对致病菌的抑制作用。结果:金属硫蛋白对羟基自由基及DPPH自由基清除能力明显强于VC,但对超氧阴离子自由基的清除效果却低于VC,金属硫蛋白对金黄色葡萄球菌、李斯特氏菌具有一定的抑制作用。金属硫蛋白清除自由基关系为Zn-MT>MT-Ⅰ>MT-Ⅱ。结论:金属硫蛋白具有较强的清除自由基能力,对部分致病菌具有一定的抑制作用,作用效果因金属硫蛋白来源、纯度及构型而不同。Objective=Scavenging free radical and antibacterial activities in vitro of two metallothionein isomeric forms from yeast and metallothioneins from animal were compared.Methods：Measuring the free radical clearance rate of metallothioneins and compared with that in control group（Vc）by UV.The antibacterial activities of metallothioneins against staphylococcus aureus, E. coil and listeria were discussed. Results： The hydroxyl and DPPH radical scavenging capacity of metallothioneins were stronger than Vc, but the superoxide anion radical scavenging capacity of metallothioneins were less than Vc.And metallothioneins also had the inhibiting effect on staphylococcus aureus and bacteria listeri＆The scavenging free radical were Zn-MT 〉 MT- Ⅰ〉 MT- Ⅱ. Conclusions= Metallothioneins had powerful capacity on scavenging free radical scavenging and certainly antibacterial action on some pathogens, the effects were different because of metal sulfur protein sources, purity and configuration.

关 键 词：酵母源金属硫蛋白 自由基 抑菌活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]