香芹酚对大肠杆菌和金黄色葡萄球菌细胞膜的影响  

Effects of carvacrol on Escherichia coli and Staphylococcus aureus membrane

作　　者：谢强[1] 林玉桓[1] 苗淑萍[1] 李晶[1] 夏秀华[1] 

机构地区：[1]无锡商业职业技术学院,江苏无锡214153

出　　处：《食品工业科技》2014年第23期54-58,62,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金青年基金(31101345)

摘　　要：研究香芹酚对革兰氏阴性菌大肠杆菌（ATCC 8735）和革兰氏阳性菌金黄色葡萄球菌（ATCC 3101）的形态、细胞膜的完整性与通透性的影响。结果表明：与对照组相比,经香芹酚处理后的样品组具有较高的紫外吸光值、电导率值和酶活力。随着香芹酚作用时间的延长,实验组的紫外吸光值、电导率值和酶活性均有快速的增加,并达到一个相对稳定的状态。同时,扫描和透射电镜图显示,经香芹酚作用6~8h后,大肠杆菌和金黄色葡萄球菌的形态均有明显改变。香芹酚在最低抑菌浓度（MIC 0.31mg/m L）作用于大肠杆菌和金黄色葡萄球菌,能够破坏菌体细胞膜和细胞壁的完整性、并引起细胞膜通透性和菌体形态的改变。This study aimed to investigate the effect of carvacrol on the morphology,cell membrane integrity and permeability of Gram-negative Escherichia coil（ ATCC 8735 ）and Gram-positive Staphylococcus aureus（ ATCC 3101）.Most samples from both strains exposed to carvacrol showed higher UV absorptions, conductivity values, and β-galactosidase activities compared with the control group and displayed a rapid rise trend.Thereafter,the values stabilized at a relatively steady state.Transmission and scanning electron microscopy images of bacterial cells exposed to carvacrol showed marked ultrastructural changes after 6 ~ 8h of exposure. The results of the present study demonstrated that carvacrol was capable of leading to the loss of cell walt and cytoplasmic membrane integrity,causing marked changes in the membrane permeability and cell morphology of Scoff and S. aureus at minimum inhibitory concentration （ MIC 0.3 ] mg/mL） concentration.

关 键 词：香芹酚 细菌细胞膜 完整性 通透性 形态结构 

分 类 号：TS201.1[轻工技术与工程—食品科学]