三种食源性致病菌挥发性代谢产物谱快速检测研究  

Study on the rapid detection of volatile metabolite spectrums of3 kinds of food- borne pathogens

作　　者：操庆国[1,2] 郭钦[2] 刘涵[2] 徐海棠[2] 李东成[2] 

机构地区：[1]江苏农林职业技术学院生物工程系,江苏句容212000 [2]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品工业科技》2014年第23期290-297,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31201373);江苏省自然科学基金资助项目(BK2010350);江苏大学人才启动基金(1281360012)

摘　　要：主要采用固相微萃取-气质联用(SPME-GC-MS)技术,检测了副溶血弧菌、大肠杆菌和金黄色葡萄球菌三种食源性致病菌的挥发性代谢产物谱(MVOCs),研究其变化规律和代谢特征性产物。结果表明,65μm PDMS/DVB纤维头萃取30min效果最好,三种致病菌MVOCs谱随培养时间延长而变化,其中,两株副溶血弧菌出峰数在30h达到最大值,可检出数分别为28和31个,特征性成分为2-氯-4-(4-甲氧基苯基)-6-(4-硝基苯基)嘧啶、二叔丁基过氧化氢、3-甲基-1-丁醇等;大肠杆菌和金黄色葡萄球菌在12h和8h出峰数最多,可检出数为42和47个,特征性成分分别为环十二烷、2-甲基-1-丁醇和吲嗪、3-甲基丁酸和4-甲氧基苄腈等。实验结果为寻找三种食源性致病菌挥发性标志物和快速检测提供研究基础。In this paper,the volatile metabolite spectrum（MVOCs） of Vibrio parahaemolyticus, Escherichia coil and Staphylococcus aureus were detected by the combination of SPME-GC-MS techniques.And the change trends of MVOCs and unique metabolites were also studied.The results showed that,the 65μm PDMS/DVB fiber head had best extraction effectin 30min and the MVOCs spectrum of 3 kinds of pathogens varied with the incubation time.The peak numbers of V.parahaemolyticus reached the maximum in 30h, which was 28 and 31, respectively and the characteristic ingredient was 2 - chlero - 4 - （ 4 - methoxyphenyl ） - 6 - （ 4 - nitrophenyl ） pyrimidine, tert - butyl hydroperoxide and 3-methyl-i-butyl alcohol.The number of peaks of Ecofi and S.aureus reached the maximum in 8h and 12h,which were 47 and 42,and the the characteristic ingredient were cyclododecane,2-methyl-1-butanol and indolizine,3-methyl-butyric acid and 4-methoxy-benzonitrile, respectively.The results provided the basis for volatile markers and rapid detection of three foodborne pathogens.

关 键 词：固相微萃取-气质联用 食源性致病菌 挥发性代谢产物谱 快速检测 

分 类 号：TS207[轻工技术与工程—食品科学]