胶体几丁质诱导红曲霉产几丁质酶的研究  

Induction effect of colloidal chitin to chitinase production by monascus

作　　者：郑天柱[1] 张庆庆[1] 胡俊[1] 谭胜[1] 蒋汶 

机构地区：[1]安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖241000

出　　处：《安徽工程大学学报》2014年第4期1-3,14,共4页Journal of Anhui Polytechnic University

基　　金：国家大学生创新创业训练计划基金资助项目(201310363021);芜湖市重点科技计划基金资助项目(芜科计字[2009]190号文)

摘　　要：本文采用胶体几丁质为底物,研究了红曲霉液态发酵产几丁质酶.研究结果表明:胶体几丁质的添加能有效诱导红曲霉合成几丁质酶.在培养基中添加0.8%(W/V)胶体几丁质的同时,添加0.4%(W/V)葡萄糖,有利于红曲霉的生长以及几丁质酶的合成,发酵48h几丁质酶活力达到最高的0.3504U/mL.The study aimed to explore the chitinase production by Monascus liquid ferment-ation.The chitinase enzyme assay was carried out with colloidal chitin as a substrate.The experimental results indi-cate that addition of colloidal chitin helps the accumulation of chitinase.The highest activity of chitinase of 0.350 4 U/mL was obtained at 48 hours in themedium with the glucose 0.4%（W/V）and colloidal chitin 0.8%（W/V）.

关 键 词：液态发酵 红曲霉 胶体几丁质 几丁质酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]