龙眼皮原花青素提取工艺优化及其抗氧化活性测定  

Optimized Extraction and Antioxidant Activity of Proanthocyanidins from Longan Pericarp

作　　者：黄尚荣[1] 杨雪娜[1] 张露[1] 郑明星[1] 谢金盛 付才力[1] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350108

出　　处：《食品科学》2014年第10期68-75,共8页Food Science

基　　金：福建省自然科学基金项目(2012J05056);福建省教育厅科技项目(JA12032);福州大学校人才基金项目(1164)

摘　　要：以龙眼果皮为原料提取原花青素。在单因素试验的基础上,采用响应曲面法研究提取时间、乙醇体积分数、液料比和提取温度对龙眼皮原花青素得率的影响,并对其进行优化。得到最佳提取工艺为提取时间25 min、液料比17∶1(mL/g)、乙醇体积分数51%、提取温度50℃,此条件下的原花青素得率为1.21%。在利用Sephadex LH-20凝胶柱层析对原花青素进行纯化后,通过1,1-二苯基-2-三硝基苯肼(DPPH)、2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除实验和还原力测定,结果显示龙眼皮原花青素具有较高的抗氧化活性。The extraction of proanthocyanidins from longan pericarp was optimized using response surface methodology. The effects of extraction time, solvent-to-solid ratio, ethanol concentration and extraction temperature on the yield of proanthocyanidins were investigated. The results showed that the optimal extraction conditions were as follows： an extraction time of 25 min, a ratio of liquid to material of 17：1 （mL/g）, an ethanol concentration of 51% and a temperature of 50 ℃. Under these conditions, the yield of proanthocyanidins was 1.21%. The extract was then purified by Sephadex LH-20 column. The purified proanthocyanidins had high antioxidant activity as indicated by scavenging activities against, 1,1-diphenyl-2-picrylhydrazyl （DPPH） and 2,2＇-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt （ABTS） radicals and reducing power.

关 键 词：龙眼皮 原花青素 响应曲面法 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]