两种胡桃醌脂质体包封率测定方法研究  

Comparison research on two methods for determining entrapment efficiency of Juglone liposome

作　　者：罗国伟[1] 白春清[2] 熊华[1] 赵强[1] 雷莉[1] 徐丽青[1] 张建飞[1] 胡振灜 范婷[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]江西科技师范大学生命科学学院,江西南昌330013

出　　处：《食品工业科技》2015年第4期146-150,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金委员会资助项目(31160317)

摘　　要：目的：建立两种胡桃醌脂质体包封率的测定方法,并对测定结果进行比较。方法：分别采用透析法和葡聚糖凝胶色谱柱法对脂质体中的游离药物进行分离,然后使用紫外分光光度计对脂质体包封的药物进行测定进而计算包封率。结果：透析法透析14h达到稳定;葡聚糖凝胶色谱柱法在30min内即将游离药物与脂质体进行有效的分离。胡桃醌测定波长为425nm,在0.005~0.04mg/m L的浓度范围内,线性关系良好,经回收率实验、精密度及重现性实验证实两种方法可行。结论：对比两种方法可知透析法耗时较长测定的包封率值偏低,葡聚糖凝胶色谱柱法测定所需时间更短,更便捷,测出的包封率更接近真实值。Objective：Two methods for determining entrapment efficiency of juglone liposome were established by comparing the determination results of two method. Methods：Dialysis and dextran gel methods were applied to separate free drugs from liposomes followed by measuring encapsulated Juglone on a UV spectrophotometer.Results ：14h was needed for dialysis method to reach dialysis equilibrium. Free drugs could be effectively separated in 30 min by dextran gel method. The content of Juglone showed good linear relationship in the concentration range of 0. 005 ~ 0. 04 mg / m L at the wavelength of 425 nm. Through the recovery experiment,precision and reproducibility experiment confirmed that the two methods were feasible. Conclusion ： A comparision of the two methods suggested that the dialysis method was time-consuming and the resulting value was low,the dextran gel method required less time,was more convenient and the entrapment efficiency measured was closer to the true value.

关 键 词：脂质体 胡桃醌 透析法 葡聚糖凝胶色谱柱法 包封率 

分 类 号：TS201.2[轻工技术与工程—食品科学]