黑毛茶中产单宁酶真菌的筛选及产酶条件研究  

Screening of the Tannase-Producing Fungi from Raw Dark Tea and Study of Fermentation Conditions

作　　者：郑重谊[1] 王娇娇[2] 资云 张冬雪[2] 周辉[2] 

机构地区：[1]湖南农业大学研究生院,湖南长沙410128 [2]湖南农业大学食品科技学院,湖南长沙410128

出　　处：《化学与生物工程》2014年第12期24-27,共4页Chemistry & Bioengineering

基　　金：湖南省教育厅重点项目(12A063);湖南农业大学青年科学基金项目(13QN10)

摘　　要：采用富含单宁酸的平板培养基对黑毛茶中产单宁酶的真菌进行筛选,经纯化后初步鉴定该菌为黑曲霉,并对其中一株黑曲霉WC-2产单宁酶的发酵条件以及发酵培养基的组成进行了优化。优化后的条件为:五倍子渣含量80%,外加碳源为α-乳糖、添加量1%,外加氮源为硝酸铵、添加量0.5%,发酵温度37℃,发酵时间72h。在此条件下,单宁酶活力最高达到27.06U·g-1。In this study,screening of the tannase-producing fungi from raw dark tea by the medium plate riched in tannins acid was investigated,and the strains were identified as Aspergillus niger.The fermentation conditions and culture medium composition of strain WC-2were optimized.The optimum conditions were as follows：dullnut dregs content of 80%,1%α-lactose as additional carbon source,0.5% ammonium nitrate as additional nitrogen source,fermentation temperature of 37 ℃,fermentation time of 72 h.Under above conditions,the enzymatic activity of tannase reached 27.06U·g^-1.

关 键 词：单宁酶 黑曲霉 固态发酵 没食子酸 

分 类 号：TQ920[轻工技术与工程—发酵工程] TS272.7[农业科学—茶叶生产加工]