蔗渣木聚糖制备低聚木糖的最优复合酶降解工艺研究  

The Research on Optimal Production Process of Xylooligosaccharides by Composite Enzymatic Hydrolysis of Bagasse Xylan

作　　者：陆登俊[1] 何亮亮 刘桂云[1] 黄琳娟[1] 李坚斌[1] 梁欣泉[1] 

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]云南省轻工业科学研究院,云南昆明650034

出　　处：《广西科学》2014年第6期610-613,共4页Guangxi Sciences

基　　金：广西科学基金项目(桂科青0832009);2011广西大学实验教学示范中心精品;特色项目建设项目资助

摘　　要：【目的】研究复合酶酶解蔗渣木聚糖制备低聚木糖的方法。【方法】采用混料试验设计中的单纯形质心方法对木聚糖酶A,木聚糖酶B和α-L-阿拉伯糖苷酶3种酶进行配方设计试验,以低聚木糖得率为评价指标。【结果】低聚木糖得率的最优酶复合配比为木聚糖酶A 39.5%,木聚糖酶B 25%,α-L-阿拉伯糖苷酶35.5%,在此条件下预测低聚木糖得率为85.20%。【结论】该配比能显著提高蔗渣木聚糖酶解效率和低聚木糖的产率。Objective]The production of xylooligosaccharides was studied by composite enzymat-ic hydrolysis of bagasse xylan.[Methods]The formulation of xylanase A,xylanase B and alpha-L-arabinofuranosidase was opitimized through simplex-centroid method for mixture design ex-periments,with the XOS extraction rate as evaluation index.[Results]The best formula was de-termined as：Xylanase A 39.5%,xylanase B 25% and alpha-L-arabinofuranosidase 35.5%.Un-der these conditions,the extraction rate of XOS was 85.20%.[Conclusion]The optimization rate of compound enzyme has the characteristic of increasing yield and efficiency.

关 键 词：复合酶 蔗渣木聚糖 低聚木糖 蔗渣 

分 类 号：TS245.9[轻工技术与工程—制糖工程]