黄海海燕体壁酶解物抗氧化活性研究  

Study on preparation of body wall hydrolysate of starfish Asterina pectinifera and antioxidant activity detection

作　　者：李裕博 纪晓琳[1,2] 刘山[1,2] 查越 辛丘岩[1,2] 启航[1,2] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]国家海洋食品工程技术研究中心,辽宁大连116034

出　　处：《食品与机械》2015年第1期137-140,共4页Food and Machinery

基　　金：国家海洋食品工程技术研究中心立项建设项目(编号:2012FU125X03);辽宁省农业领域青年科技创新人才培养计划(编号:2014001)

摘　　要：分别用胃蛋白酶、中性蛋白酶和胰蛋白酶在各自最适条件下酶解黄海海燕体壁制得酶解物,并应用电子自旋共振波谱技术检测酶解物对DPPH和羟基自由基的抗氧化活性。结果表明,黄海海燕体壁酶解物对DPPH和羟基自由基的清除能力随浓度升高而增强;酶解物浓度在20 mg/mL时,胃蛋白酶组、中性蛋白酶组和胰蛋白酶组对DPPH自由基的清除率分别为(52.25±0.14)%,(68.30±0.37)%,(46.27±0.47)%,对羟基自由基的清除率分别为(72.61±0.17)%,(81.82±0.79)%,(56.77±0.28)%;酶解物具有较明显的抗氧化能力。Hydrolysate was extracted from starfish Asterina pectinifera,the starfish Asterina pectiniferas were hydrolyzed with pepsin,neutrase and trypsin under their optimal conditions to obtain the hydrolysate,and the antioxidant activity of hydrolysate was detected by using electron spin resonance（ESR）spectroscopy.The results indicated that the DPPH radical and hydroxyl radical scavenged ability increased with the increasing concentration of hydrolysate;at the concentration of 20mg/mL,the clearance rate of pepsin group,neutrase group and trypsin group on DPPH radical were（52.25±0.14）%,（68.30±0.37）% and（46.27±0.47）% respectively,and the rate of hydroxyl radical were（72.61±0.17）%,（81.82±0.79）% and（56.77±0.28）% respectively.

关 键 词：黄海海燕 酶解物 抗氧化活性 电子自旋共振 自由基 

分 类 号：TS201.25[轻工技术与工程—食品科学]