海胆共附生菌DNC_2的鉴定及其发酵条件优化  

Identification andoptimization of fermentation conditions for symbiotic and epiphyte bacterium DNC_2 from Strongylocentrotus intermedius

作　　者：李振[1] 张付云[1] 李伟[1] 赵宇[1] 卢航[1] 

机构地区：[1]大连海洋大学食品科学与工程学院,辽宁大连116023

出　　处：《食品与机械》2015年第1期146-150,共5页Food and Machinery

基　　金：辽大连市科学技术基金(编号:2010J21DW017);大连海洋大学2012年校列科研项目(编号:2012HYDX15);大学生创新创业训练项目(编号:201310158204)

摘　　要：为对海胆共附生菌DNC2进行开发利用,对该菌株进行形态、生理生化以及16SrDNA序列测定,并以抑菌活性为考察指标,对其发酵条件进行优化。结果表明:海胆共附生菌DNC2属于枯草芽孢杆菌(Bacillus subtilis);用牛肉膏为0.3%,蛋白胨为0.6%,初始pH值9.0的培养基,35℃发酵5d时抑菌活性最优,且该条件的菌株发酵液对8种真菌指示菌抑菌效果良好,抑制率分别为苹果粉红92%,玉米早疫、番茄灰霉89%,辣椒青枯88%,毛霉菌81%,西瓜枯萎73%,棉花枯萎72%,串珠镰刀菌52%。To explore the Symbiotic and Epiphyte bacterium DNC2 fromStrongylocentrotus intermedius,the morphological,biochemical and physiological characteristics of the strain were researched and the 16 SrDNA sequences of it were analyzed.The univariate experiment and orthogonal test of L16（45）were carried out to optimize the fermentation conditions for antibacterial activity.Symbiotic and Epiphyte bacterium DNC2 was identified as Bacillus subtilis.The optimal fermentation conditions were obtained as fermentation at 35 ℃for 120 hwith initial pH 9,and the optimum fermentation medium was determined as beef extract 0.3% and peptone 0.6%.The optimized fermentation broth also showed good effect on inhibiting the growth of 8kinds of fungus.The inhibition rate was 92% against Trichothecium roseum（Bull.）Link,89% against Botrytis cinerea Pers and A.solani（EllMart）.sorauer,88% against Ralstonia solanacearum,81%against Mucor mucedo（L.）Brefeld,73%against Fusarium oxysporum f.sp.niveum,72% against Fusarium oxysporum of Cotton and 52% against Fusarium moniliformis.

关 键 词：海胆 共附芽孢杆菌 鉴定 抑菌活性 发酵条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]