不同质量窖泥古菌群落的研究  

Archaea Communities in Pit Mud of Different Quality

作　　者：杜礼泉[1,2] 罗惠波[3] 黄治国[3] 苟云凌[2] 贺新生[1] 王远成[2] 饶家权[2] 王晓刚[2] 范昌明[2] 唐聪[2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省绵阳市丰谷酒业有限责任公司,四川绵阳621000 [3]四川理工学院生物工程学院,四川自贡643000

出　　处：《酿酒科技》2015年第2期58-61,共4页Liquor-Making Science & Technology

摘　　要：采用多聚酶链式反应结合变性梯度凝胶电泳指纹分析技术(PCR-DGGE)对不同质量窖泥中古菌群落结构进行分析,说明了不同质量窖泥古菌种群的复杂性,再次印证了产甲烷菌是窖泥中古菌的优势菌,并在此基础上揭示了窖泥中产甲烷菌种属上的多样性。发现了以最优质窖泥为参照,窖泥质量与其古菌群落间的相似性指数总体呈正相关这一规律,为今后研究窖泥培养、养护和应用提供了理论依据。In this study, the structures of archaea communities in pit mud of different quality were analyzed by PCR-DGGE. The analytic re-sults proved the complexity of archaea communities in pit mud of different quality. And it also proved that methanogenic bacteria was the domi-nant archaeal in pit mud. Besides, the analytic results revealed the diversity of methanogenic bacteria species. It was found that, with reference to the best pit mud, the quality of pit mud was positively correlated to archaeal community similarity indexes. Those findings provided theoreti-cal evidences for further research on pit mud culture, maintenance and application.

关 键 词：微生物 浓香型白酒 窖泥 古菌群落 白酒 

分 类 号：TS261.1[轻工技术与工程—发酵工程]