分离传统酸马奶中乳酸菌的最适培养基的筛选  

Screen of the optimum medium for isolation of lactic acid bacteria in traditional koumiss

作　　者：德亮亮 乌仁图雅[1] 秦艳婷[1] 白娜[1] 扎木苏[1] 陈红霞[1] 刘文俊[1] 张和平[1] 孟和毕力格[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国乳品工业》2014年第8期4-7,共4页China Dairy Industry

基　　金：国家863计划项目(国家高技术研究发展计划(863计划));内蒙古自然科学基金项目(国家自然科学基金)

摘　　要：以采集于新疆地区的10份酸马奶样品为研究对象,通过对比5种(MRS培养基、MRS合成培养基、M17培养基、MRS+X-gal培养基和MRS+蘑菇汁+番茄汁培养基)不同的培养基上进行乳酸菌的活菌计数,且分析分离株统计结果筛选出最适于乳酸菌的生长、分离的培养基。实验表明,MRS合成培养基更加适合乳酸菌的生长,其活菌数最高,便于乳酸菌计数。Ten samples of koumiss collected from Xinjiang Province in China was used as the research object. To screen the most optimum medium of the growth and isolation of lactic acid bacteria in this study. five different medium（MRS agar, MRS synthetic agar, M17 agar,MRS＋X-gal agar and MRS＋ mushroom juice＋ tomato juice agar） were used to isolate and enumerate the lactic acid bacteria. Based on the viable counts and species statistics analysis of the isolates, the results showed that MRS synthetic medium is was more suitable for the growth of lactic acid bacteria, with the highest number of living bacterium counts.

关 键 词：酸马奶 乳酸菌 活菌数 培养基筛选 

分 类 号：Q93-335[生物学—微生物学]