木耳黑色素的发酵制备及其清除自由基活性研究  

Production of Melanin by the Fermentation of Auricularia auricula and Its Free Radical Scavenging Activity

作　　者：张敏[1] 陈燕璐 程菲菲[1] 卞丹[1] 求路慧 杨海龙[1] 

机构地区：[1]温州大学生命与环境科学学院,浙江温州325035

出　　处：《核农学报》2015年第2期304-312,共9页Journal of Nuclear Agricultural Sciences

基　　金：浙江省新苗人才计划项目(2013R424028)

摘　　要：黑色素具有抗氧化、清除自由基、刺激免疫等一系列的生物活性。本文以深层发酵法制备木耳黑色素,并对木耳黑色素进行体外抗氧化作用研究,考察了碳、氮源、金属离子对木耳菌产黑色素的影响,用Box-Behnken设计结合响应面分析法优化了发酵培养基组成。结果表明,最佳发酵培养基为:酪蛋白0.747%、可溶性淀粉1.726%、Ca CO30.140%,在此条件下木耳黑色素的产量可达170.37 mg·L-1。在一定范围内,发酵制备的木耳黑色素具有较强的清除超氧阴离子自由基和ABTS阳离子自由基能力。木耳黑色素是一个天然和有效的抗氧化剂,深层发酵是生产这一活性物质的有效方法。本研究为木耳黑色素的工业发酵生产奠定了基础。Melanin has many bio-activities, including antioxidant, free radicals-scavenging, immuno-improving, etc. Melanin was produced by submerged culture of Auricularia auricula and its antioxidant activities were also researched in this paper. The effects of carbon sources, nitrogen sources and metal ions on the melanin production by A. auricula were firstly studied by one-factor-at-a-time method, and then the media components were optimized by response surface methodology based on Box-behnken experiment design. The results showed the optimal media for melanin production contained, casein, 0.747%, soluble starch, 1.726%, CaCO3, 0. 140% and the melanin concentration reached 170.37 mg·L-1 under the optimal conditions. Melanin produced by fermentation of A. auricula has strong O2-· and ABTS ~＂ scavenging activity and it is a natural and effective antioxidant. The result from this work are helpful melanin productcon by submerged culture.

关 键 词：木耳 黑色素 发酵 响应面 清除自由基活性 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TS264.4