黄鳍金枪鱼皮胶原肽酶解工艺及抗氧化活性研究  

Studies on Enzymolysis Technology of Collagen Peptide and Antioxide Activities from Tuna Skin

作　　者：王雨生[1,2] 冷云[1] 陈海华[1] 王慧云[1] 

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]青岛农业大学学报编辑部,山东青岛266109

出　　处：《中国食品学报》2015年第2期72-78,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省高等学校优秀中青年骨干教师国际合作培养项目(SD2013-8-25)

摘　　要：以金枪鱼皮为原料,以肽得率、水解度和感官评价为指标,确定金枪鱼皮胶原肽酶解的最优工艺条件是:中性蛋白酶,酶解时间4 h,酶添加量1 000 U/g,此时肽得率29.03%。体外抗氧化能力测定结果:金枪鱼皮胶原蛋白酶解物具有一定的抗氧化活性,总还原力是VC总还原力的60%;与VC相比,DPPH·清除率(IC50=0.43 mg/m L)、O22-清除率(IC50=3.67 mg/m L)、卵磷脂过氧化物清除率(IC50=14.67 mg/m L)较低,·OH清除率(IC50=0.39 mg/m L)、H2O2清除率(IC50=0.05 g/m L)较高。该酶解物能显著降低油脂的过氧化值,然而降低幅度略低于VC。The optimum enzymolysis technology of collagen peptide from tuna skin was investigated by single factor experiment according to the peptide yield, hydrolysis degree and sensory evaluation. The result showed that the enzymolysis conditions were enzymolysis time of 4 h, enzyme addition of 1 000 U/g, under which the peptide yield was 29.03%.The antioxide activities of tuna skin hydrolysate in vitro showed that reducing power was 60% of VC. DPPH· radical scavenging rate（IC50=0.43 mg/m L）, O22-scavenging rate（IC50=3.67 mg/m L）, lecithin superoxide scavenging rate（IC50=14.67 mg/m L） of the tuna skin hydrolysate was lower than that of VC, while ·OH radical scavenging rate（IC50=0.39 mg/m L）, H2O2 scavenging rate（IC50=0.05 g/m L） was higher than that of VC. In addition, the POV of oil treated with tuna skin hydrolysate was slightly lower than that treated with VC.

关 键 词：金枪鱼皮 胶原蛋白肽 酶解 肽得率 抗氧化活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]