紫外结合亚硝酸诱变选育中性蛋白酶高产菌株  

Study on the screening high yield neutral-protease-producing strain by combing UV and nitrous acid mutagenesis

作　　者：唐雪鹭[1,2] 崔春[1,2] 雷芬芬[1,2] 舒会[1,2] 赵谋明[1,2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]广东省食品绿色加工与营养调控工程技术研究中心,广东广州511400

出　　处：《食品工业科技》2015年第7期163-166,共4页Science and Technology of Food Industry

基　　金：深海微生物活性物质挖掘与其利用技术(2012AA092104);广东省海洋经济创新发展区域示范专项(GD2012-D01-002);热带海洋微生物新型耐盐特异性蛋白酶的挖掘与应用(2013418018-7)

摘　　要：为提高一株深海来源微小杆菌属细菌Exiguobacterium sp.SWJS2产中性蛋白酶的酶活,本文先后采用紫外线和亚硝酸对其进行诱变处理。紫外诱变条件为8W紫外灯在20cm处直接照射50s;三轮诱变后得突变株UV-48,其酶活较原始菌株提升21.32%。再以UV-48为出发菌株进行亚硝酸诱变,条件为0.03mol/L亚硝酸作用8min;三轮诱变后得突变株HN-34,其酶活为1109U/m L,较突变株UV-48提升13.97%,较原始菌株提升38.28%。HN-34传代5次相对酶活均在94%以上,遗传性状稳定。UV and HNO2 were successively used to promote the enzyme-activity of the neutral protease produced by a marine bacteria Exiguobacterium sp.SWJS2.Being directly exposed to 8W UV for 50s with a vertical dimension of 20cm, mutant strain UV-48 with a promotion of 21.32% were obtained after three turns of mutagenesis and then be applied into HNO2 mutagenesis for further promotion. Being diluted by 0.03mol/L HNQ2 for 8rain, UV-48 was finally turned into HN-34 with a promotion of 13.97% than itself and 38.28% than the starting strain after three turns. After pass-generation tests for five times,the mutant strain HN-34 was proved to be genetically stable since the five relative enzyme-activity were all above 94%.

关 键 词：中性蛋白酶 诱变 紫外 亚硝酸 微小杆菌属 

分 类 号：TS201.3[轻工技术与工程—食品科学]