油茶籽壳提取物抗氧化及抗癌活性研究  

In vitro antioxidant and antiproliferative activities of the different extracts from shell of Camellia oleifera Abel

作　　者：淦永鉴 李旭[1] 杨莉琳[1] 丁春邦[1] 曾宪垠[1] 张怀渝[1] 陈志渝[1] 杜小刚[1] 

机构地区：[1]四川农业大学生命科学学院,四川雅安625014

出　　处：《食品工业科技》2015年第8期171-174,182,共5页Science and Technology of Food Industry

基　　金：四川省肉类加工实验室开放基金(13-R04)

摘　　要：目的：观察油茶籽壳不同溶剂提取物（乙酸乙酯、正丁醇、氯仿、水）体外抗氧化和抗肿瘤活性。方法：采用Folin-Ciocalteu和三氯化铝方法检测总酚及总黄酮含量;采用DPPH法和邻二氮菲-Fe2＋氧化法测抗氧化活性;采用CCK-8法测细胞的活性。结果：油茶籽壳乙酸乙酯提取物、正丁醇提取物、氯仿提取物、水提取物总酚含量分别为79.25±4.552、51.81±3.651、2.762±0.523、19.73±1.191mg/g;总黄酮含量分别为26.73±0.613、15.63±1.126、13.16±0.672、5.734±0.761mg/g;油茶籽壳乙酸乙酯提取物、正丁醇提取物、氯仿提取物、水提取物在15.625~250μg/m L范围内体外抗氧化活性均具有剂量依赖性;对Hep G2细胞增殖抑制作用在62.5~250μg/m L范围内均具有剂量依赖性。结论：体外抗氧化活性和抗肿瘤活性强弱均表现为乙酸乙酯提取物〉正丁醇提取物〉水提取物〉氯仿提取物,其中乙酸乙酯是油茶籽壳提取的有效溶剂。Objective：The paper investigated antioxidant and antiproliferative properties of various extracts（Ethyl Acetate,N-butanol,Chloroforms,Aqueous） from the shells of Camellia Oleifera Abel. with in vitro. Methods：Total phenolics and total flavonoids were measured by Folin- Ciocalteu reagent and aluminium chloride reagent,respectively. And,the antioxidant activity was tested by DPPH and hydroxyl assays in vitro. Besides,the proliferative activity of the different human cancer cell lines was detected by CCK-8 assay. Results ：Total phenolic contents of extracts（Ethyl Acetate,N-butanol,Chloroforms,Aqueous） were（79.25 ±4.552）mg/g,（51.81±3.651）mg/g,（2.762±0.523）mg/g,（19.73±1.191）mg/g respectively,and total flavonoid contents were（26.73±0.613）mg/g,（15.63±1.126）mg/g,（13.16±0.672）mg/g,（5.734±0.761mg/g,respectively. The antioxidant activities of these extracts in a range of concentrations from 15.625 to 250μg/m L increased in dose-dependent manner. More over,the antiproliferative activities of these extract in a range of concentrations from 62.5 to250μg/m L increased in dose-dependent manner. Conclusion：Antioxidant and antiproliferative activities strength of Ethyl acetate extracts〉N-butanol extracts〉Aqueous extracts〉Chloroform extracts in vitro,Ethyl acetate was an effective solvent to extract shell of Camellia oleifera Abel.

关 键 词：油茶籽壳 提取物 抗氧化活性 抗肿瘤活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]