多粘类芽孢杆菌HD-1产纤维素酶的条件优化  

Optimization of producing conditions of the cellulase from Paenibacillus polymyxa HD-1

作　　者：程爱芳[1] 邓政东[1] 陈文[1] 周念波[1] 黄芳一[1] 

机构地区：[1]武汉生物工程学院生命科学与技术学院,湖北武汉430415

出　　处：《食品工业科技》2015年第10期173-177,共5页Science and Technology of Food Industry

基　　金：武汉生物工程学院校级研究项目(2013JYI01)

摘　　要：采用DNS比色法,通过单因素实验和正交实验对多粘类芽孢杆菌HD-1产纤维素酶的培养基主要成分和培养条件进行优化。结果表明:该菌种产纤维素酶的最适培养基为麸皮0.5%、酵母膏1.25%、磷酸二氢钾0.04%、硫酸镁0.1%,培养基初始p H8.0;最适培养条件为接种量4%,培养温度35℃,培养时间51h。在此优化条件下,多粘类芽孢杆菌产纤维素酶活性可达88.3U/m L。In order to improve the production of cellulase,the fermentation medium and culture conditions for Paenibacillus polymyxa HD-1 were optimized by single factor test and orthogonal test. The results showed that the best compositions of fermentation medium were 0.5% of bran, 1.25% of yeast extract,0,04% of KH2PO4.3H20, 0.1% of MgSO4.7H20,with the initial pH of the medium was 8.0;and the best fermentation inoculum size was 4% ,culture temperature at 35℃ and culture time for 51 hours. Under this optimal conditions,the activity of cellulase from Paenibacillus polymyxa HD-1 could reach 88.3U/mL.

关 键 词：多粘类芽孢杆菌HD-1 纤维素酶 发酵条件 优化 

分 类 号：TS202.3[轻工技术与工程—食品科学]