CO_2超临界萃取技术对金枪鱼油挥发性成分的分析  

The Analysis of the Effect of Supercritical CO_2 Extraction Technology on Volatile Component of Tuna Oils

作　　者：王亚男[1] 季晓敏[1] 黄健[2] 王求娟 陈义芳 夏静波 苏秀榕[1] 

机构地区：[1]宁波大学海洋学院,宁波315211 [2]北京普析通用仪器有限责任公司,北京101200 [3]宁波今日食品有限公司,宁波315502

出　　处：《中国粮油学报》2015年第6期74-78,100,共6页Journal of the Chinese Cereals and Oils Association

基　　金：宁波市科技局农业与社发重大科技项目(2010C10040)

摘　　要：采用超临界CO2萃取技术精制金枪鱼粗鱼油,依据现有对油脂氧化程度的评价指标:过氧化值(POV)和酸值(AV),运用电子鼻和顶空固相微萃取-气质联用法对不同萃取条件下的鱼油进行分析。结果表明,超临界CO2精制的鱼油酸值和过氧化值符合水产行业标准对精制鱼油的要求,32℃,20 MPa酸值最小,30 MPa过氧化值最小。电子鼻能够快速、灵敏的检测到鱼油中挥发性物质的变化,通过PCA和LDA方法分析原鱼油、32℃,20 MPa、32℃,30 MPa和40℃,20 MPa条件精制的鱼油挥发性物质明显不同,利用GC-MS分别检测出46、19、12和23种挥发性物质,包括酸类、醛类、烃类、酮类、醇类、酯类,还有少量杂环化合物、胺类等。超临界CO2流体萃取技术对醛类、酮类、胺类等主要影响鱼油风味的物质去除率达到100%,对酸类去除效果良好。Based on the existing evaluation indexes of oil oxidation degree, supercritical CO2 extraction technol- ogy was used to refine crude fish oil. Electronic nose and headspace - solid phase micro - extraction （ HS - SPME） coupled with gas chromatography - mass spectrometry （ GC - MS） were used to analyze the volatiles of tuna fish oil which was extracted differently. The results showed that the AV and POV of the products of fish oil met the requirements of industry standard, and the AV was the lowest by 32 ℃ ,20 MPa ,the POV was the lowest by 32 ℃ ,30 MPa and 40 ℃ , 20 MPa, and electronic nose can be sensitive to detect the volatiles change of fish oil. The volatiles of crude fish oil which were tested by 32 ℃ ,20 MPa,32 ℃ ,30 MPa and 40 ℃ ,20 MPa were significantly different by PCA and LDA methods. There were 46, 19, 12, and 23 kinds of volatile compounds including acids, aldehydes, ketones, and hydrocarbons, alcohols, esters, as well as a number of heteroeyelie compounds, amines. The odor which may affect fish oil including aldehydes, ketones, amines, etc, were removed up to 100% by using supercritical CO2 extraction technology, besides, the acids were removed efficiently.

关 键 词：超临界CO2 鱼油 精制 电子鼻 挥发性物质 

分 类 号：TQ646[化学工程—精细化工]