液态烷烃氧载体对褐黄孢链霉菌合成纳他霉素的影响  

Effects of oxygen vector liquid alkanes on natamycin production by Streptomyces gilvosporeus

作　　者：王爽[1] 张岩[1] 王赞[1] 李永波[1] 章晶晶[1] 马超峰[1] 周巍[1,2] 

机构地区：[1]河北省食品检验研究院河北省食品安全重点实验室 [2]河北农业大学食品科技学院,河北保定071000

出　　处：《食品与发酵工业》2015年第1期11-17,共7页Food and Fermentation Industries

基　　金：河北省质量技术监督局科技计划项目(100107)

摘　　要：利用单因素摇瓶发酵的实验方法,以多种液态烷烃作为氧载体,分别研究了其不同添加浓度、添加时间对褐黄孢链霉菌生长及对纳他霉素生物合成的影响。实验结果表明,正己烷、正十二烷和正十六烷都不能作为碳源被细胞利用,是有效的氧载体。正十二烷作为氧载体效果较好:接种同时添加6%的正十二烷培养,细胞干重和纳他霉素含量分别比对照组提高了22.9%和46.4%;而最佳添加时间为发酵后24 h,纳他霉素含量比接种同时添加提高了7.0%。添加6%的正十六烷,纳他霉素含量比对照组提高了43.3%。添加高于3%浓度的正己烷,菌体干重和那他霉素产量均低于对照组,推测可能是正己烷对细胞产生毒性而抑制了菌体生长和纳他霉素的合成。Oxygen supply plays a major role for Streptomyces gilvosporeus growth and biosynthesis of natamycin. The effects of several liquid alkanes, which were used as organic oxygen vectors, on Streptomyces gilvosporeus growth and biosynthesis of natamycin were investigated. Shaking flask fermentation with single factor comparison experiment was adopted. It was found that the n-hexane, n-dodecane and n-hexadecane were useful oxygen vector, which could not be used as carbon source. The result showed that n-dodecane was the most suitable oxygen vector and the optimum concentration was 6% （V/V） , while the maximumu biomass and the natamycin production were improved by 22.9% and 46.4% respectively compared with control group. The optimum time was 24 hour, and the natamycin production was improved by 7.0%. With the addition of 6% n-hexadecane, the natamycin production was improved by 43.3% compared with control group. Higher concentration of n-hexane inhibited cell growth and biosynthesis of natamycin be- cause of its cytotoxicity.

关 键 词：褐黄孢链霉菌 纳他霉素 氧载体 液态烷烃 

分 类 号：TQ465[化学工程—制药化工]